Capillary Electrophoresis – Mass Spectrometry (CE–MS): Principles and Applications

This monograph offers the reader a complete overview on both principles and applications of CE–MS. Starting with an introductory chapter on detection in CE, also related and more specialized techniques such as electrophoretic and chromatographic preconcentration are discussed. A special emphasis is put on CE–MS interfaces, which are described in detail. In a separate chapter, attention is paid to sheath–liquid interfacing. The developments and possibilities of microchip CE–MS are also described. Applications to all relevant areas are discussed in distinct chapters, each written by experts in the respective fields. Besides applications in pharmaceutical analysis and bioanalysis, recent implementations in food science, forensic analysis, analysis of intact proteins, metabolomics and proteomics are highlighted. MS is a perfectly appropriate detection system in CE, as efficient separation is coupled to sensitive and selective detection. Moreover, MS can provide structure information on the separated compounds. CE–MS has now been developed into a strong hyphenated system complementary to LC–MS. This monograph is an unique source of knowledge for everyone dealing with and interested in CE–MS.

Detection in Capillary Electrophoresis – An Introduction

Electrospray Ionization Interface Development for Capillary Electrophoresis–Mass Spectrometry

Sheath Liquids in CE–MS: Role, Parameters, and Optimization

Recent Developments of Microchip Capillary Electrophoresis Coupled with Mass Spectrometry

On–line Electrophoretic, Electrochromatographic, and Chromatographic Sample Concentration in CE–MS

CE–MS in Drug Analysis and Bioanalysis

Intact Protein Analysis by Capillary Electrophoresis – Mass Spectrometry

CE–MS in Food Analysis and Foodomics

CE–MS in Forensic Sciences with Focus on Forensic Toxicology

CE–MS in Metabolomics

CE–MS for Clinical Proteomics and Metabolomics: Strategies and Applications

Gerhardus (Ad) de Jong studied chemistry in Utrecht, the Netherlands, and received his Ph.D. at the Free University of Amsterdam. He worked for Duphar, a pharmaceutical company in Weesp, the Netherlands, in the department Analytical Research. Subsequently, he returned to the Free University as associate professor in the Department of Analytical Chemistry. In 1990, he again moved to the pharmaceutical industry and was appointed to section head Chromatography of Solvay Duphar. In 1995, he was appointed professor in Analytical Chemistry and Pharmaceutical Analysis at the University Centre for Pharmacy, Groningen, the Netherlands, and since 2001, he is professor in Pharmaceutical Analysis at the Department of Pharmaceutical Sciences, Utrecht University.
Prof. de Jong is (co–)author of more than 300 papers in various scientific journals and some book chapters. He is co–author of a book on chromatography and was editor of the Journal of Chromatography B from 1998 to 2003. His research interests encompass separation techniques and their applications in pharmaceutical analysis, bioanalysis and analysis of biomacromolecules.