Fluorescence Microscopy: From Principles to Biological Applications

This text is specifically designed to allow advanced students and researchers with little background in physics to comprehend both microscopy basics and state–of the–art light microscopy techniques. This second edition was carefully revised and features new chapters on super resolution, 2–photon and light sheet fluorescence microscopy, making it even more suitable for teaching and training courses on modern microscopy. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to include more didactical features: Theoretical derivations have been concentrated in text boxes and summaries provide fast access for beginners. The supplementary material has been expanded, with a companion website that offers additional teaching material including videos.

1. Introduction Ulrich Kubitscheck
2. Principles of Light Microscopy Ulrich Kubitscheck
3. Fluorescence Microscopy Jurek W. Dobrucki
4. Fluorescence Labeling Gerd Ulrich Nienhaus and Karin Nienhaus
5. Confocal Microscopy Don C. Lamb
6. Fluorescence Photobleaching and Photoactivation Techniques Reiner Peters
7. Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging Fred S. Wouters
8. Multi–Photon Excitation N.N.
9. Single Molecule Microscopy in the Life Sciences Gerhard J. Schütz
10. Super–Resolution Microscopy: Interference and Pattern Techniques Christoph Cremer
11. STED Microscopy Joerg Bewersdorf
12. Light Sheet Fluorescence Microscopy N.N.
Appendix A: Practical Guide to Optical Alignment Rainer Heintzmann
Appendix B: Matrices and Images N.N.


Ulrich Kubitscheck is the department head of Biophysical Chemistry at the Institute of Physical and Theoretical Chemistry at the University of Bonn, Germany. Having obtained his academic degrees from the University of Bremen, Germany, he spent his career working at The Weizmann Institute of Science and the Institute of Medical Physics and Biophysics of the University of Münster, Germany, before taking up his present appointment at the University of Bonn. Professor Kubitscheck develops single molecule and light sheet imaging techniques, has authored over 80 scientific publications and has extensive experience in teaching courses on physical chemistry, biophysics and quantitative microscopy.