Chemical Cytometry: Ultrasensitive Analysis of Single Cells

Tying together technological and conceptual advances of the past decade, this seminal book introduces the novel approach of chemical cytometry –– the biochemical analysis of cell constituents at the single cell level. As such, it combines recently developed methods for the handling of single cells, the separation of their components, and the detection and analysis of biomolecular species with application examples from cell biology, neurobiology and molecular medicine.
The editor has succeeded in acquiring contributions from the recognized leaders in the field, resulting in a complete and authoritative overview for both developers as well as users of these new methods.


Spis treści:
Origin, current status and future perspectives of chemical cytometry
Metabolic cytometry – the study of glycosphingolipid metabolism in single primary cells of the dorsal root ganglia
Cell signaling studied at the single cell level
Ultrasensitive detection of low–copy molecules from single cells
Capillary electrophoresis of nucleic acids at the single–cell level
Microfluidic Technology for single–cell analysis
On–chip electroporation and electrofusion for single–cell engineering
Electroporative flow cytometry for single–cell analysis
Ultrasensitive analysis of individual cells via droplet microfluidics
Probing exocytosis at single cells using electrochemistry
Electrochemical determination of enzyme activity in single cells
Single cell mass spectrometry
Optical sensing arrays for single–cell analysis


Nota biograficzna:
Chang Lu is a professor of biological engineering at Purdue University, West Lafayette (USA). Having obtained his academic degrees from Peking University and from the University of Illinois at Urbana–Champaign, he received post–doctoral training at Cornell University before moving to Purdue. His current research is foc used on the application of microfluidics and spectroscopy tools to study single cells. He has received a number of awards, including the NSF career award and the Wallace H. Coulter foundation Early Career Award in Biomedical Engineering.