Proteomics in Practice: A Guide to Successful Experimental Design

A thoroughly revised edition of the successful practical guide to experimental methods in proteomics, which now includes DIGE technology and liquid chromatography.
The first part, written in a textbook style, presents the various techniques and routes required in approaching proteomic analysis. Key methods explained are electrophoretic techniques including isoelectric focusing, SDS page, 2–D page and DIGE, and liquid chromatography techniques including ion exchange, affinity chromatography and reversed–phase HPLC. Mass–spectrometric techniques such as MALDI, ESR and FT ICR are addressed as well as a section on proteome functionality. The second part is a comprehensive, laboratory manual spanning the full range of methods from sample preparation to protein identification. The third part is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further information.
Updates of protocols as well as animations illustrating crucial methodological steps are available on the Web at http://www.wiley–vch.de/books/info/978–3–527–31941–1/index.html


Spis treści:
Preface
Foreword
Abbreviations
Glossary of terms
INTRODUCTION
History
Critical points
Hypothesis driven approach vs. High Throughput
Complementarities of workflows
Concept of experimental planning
Success stories, highlights of useful references
PART I: PROTEOMICS TECHNOLOGY
Electrophoresis
Liquid Chromatography
Mass spectrometry
Bioinformatics tools / data mining
Methods under development
PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS
Strategy where to start
Gel–based workflow
LC–based workflow
Latest trends
Validation with Western blotting
Statistic evaluation
PART III: TROUBLE SHOOTING
Gel–ba sed workflow
LC based workflow
References
Index


Nota biograficzna:
Reiner Westermeier gained his doctorate in 1981 at the Technical University of Munich, Germany. There he remained engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours.
From 1984 to 1987 Reiner Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese–Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech, later GE Healthcare since July 1997, and its Senior Scientist for Proteomics since January 2002.
Reiner Westermeier is the author of several publications and two books, both published by WILEY–VCH.
Tom Naven and Hans–Rudolf Höpker are also working for GE Healthcare as proteomics scientists.

Okładka tylna:
A thoroughly revised edition of the successful practical guide to experimental methods in proteomics, which now includes DIGE technology and liquid chromatography.
The first part, written in a textbook style, presents the various techniques and routes required in approaching proteomic analysis. Key methods explained are electrophoretic techniques including isoelectric focusing, SDS page, 2–D page and DIGE, and liquid chromatography techniques including ion exchange, affinity chromatography and reversed–phase HPLC. Mass–spectrometric techniques such as MALDI, ESR and FT ICR are addressed as well as a section on proteome functionality. The second part is a comprehensive, laboratory manual spanning the full range of methods from sample preparation to protein identif ication. The third part is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further information.
Updates of protocols as well as animations illustrating crucial methodological steps are available on the Web at http://www.wiley–vch.de/books/info/978–3–527–31941–1/index.html