The Handbook of Plant Functional Genomics: Concepts and Protocols

The Present Handbook of Plant Functional Genomics (Concepts and Protocols) Spans over several aspects of current and emerging technologies in the field.

The editors – two of the leading experts in research with a proven track record – combine their expertise to provide an invaluable reference on the topic. To make it more inclusive, this second volume in the Molecular Plant Biology Handbook Series is divided into four main parts:

Transcriptome Analysis Gene Silencing, Mutation Analysis, and Functional Genomics Computational Analysis Functional Genomics and Emerging Technologies.

Each chapter introduces the subject comprehensively, and provides state–of–the art experimental protocols. A handy glossary of relevant technical terms is included to facilitate reading for researchers, teachers and students.

TRANSCRIPTOME ANALYSIS
Single–Cell Expression Profiling
AFLP–based RNA Fingerprinting
The SAGE family: SAGE, LongSAGE and SuperSAGE
DeepCAGE: High–Throughput Gene Expression Analysis for Transcription Start Site and Promoter Identification
Paired End diTagging (GIS–PET) for Transcriptome Characterization
High–Throughput Functional Screening of Genes in planta
Microarrays as Tools to Decipher Transcriptomes in Symbiotic Interactions
BeadArray–based Gene Expression Analysis
Genome–wide Analysis of mRNA expression by Fluorescent Differential Display
Quantitative Real–time RT–PCR

GENE SILENCING, MUTATION ANALYSIS, AND FUNCTIONAL GENOMICS
RNA Interference for Functional Genomics
Extending Functional Genomics: VIGS for Model and Crop Plants
Identification of Expressed Genes by Mutation: TILLING
Identification of Expressed Genes by Mutation: Transposon Tagging
Fast Neutron Mutagenesis

COMPUTATIONAL ANALYSIS
Bioinformatics Tools to Discover Co–expressed Genes in Plants
Promoter Analysis
Integration and Phylogenomics
Structural, Functional, and Comparative Annotation of Plant Genomes
Large–Scale Genomic Sequence Comparisons and Gene Identification with ClustDB

EMERGING TECHNOLOGIES
Nanotechnologies and Fluorescent Proteins for Functional Genomics
Next Generation Sequencing Technologies
454 Sequencing for Functional Genomics

Günter Kahl is Professor for Plant Molecular Biology in Frankfurt am Main, Germany. He has served in expert missions for IAEA, FAO and UNESCO across the world and is author of more than 250 scientific publications, including several Wiley–VCH books.

Khalid Meksem is Professor at the Department of Plant, Soil & General Agriculture of Southern Illinois University. He is associate editor of the Journal of Biomedicine and Biotechnology and chaired the Functional Genomic Workshop at the International Plant & Animal Genome Conference in San Diego. He is reviewer for several granting agencies, including the National Science Foundation and the US Department of Agriculture.

"... I recommend this book to any plant researcher who is interested in incorporating functional genomics approaches or who is looking to expand their repertoire. I especially liked that most chapters include detailed ′recipes′, which show that anyone with a standard wet laboratory can do most of the procedures... a very useful volume..." –The Quarterly Review of Biology, March 2010