HPLC Made to Measure: A Practical Handbook for Optimization

Alongside developing new ones, the optimization of existing methods is a key task in the HPLC laboratory. And a task that nowadays has to be solved increasingly fast and cost–efficiently.
This handbook provides well–founded assistance in better meeting this challenge. Internationally renowned authors cover both the general basics and strategies in optimization, as well as the specific aspects involved in such different methods as RP–HPLC, NP–HPLC, micro– and nano–HPLC, and hyphenated techniques, such as LC/MS. Nor are such topics as column selection and chiral separations left uncovered. Some of the contributions present applications using common optimization software, such as DryLab or ChromSword. All of the chapters concentrate on the essentials and are written in a practically oriented style, while their self–contained structure allows for targeted references.
Throughout the text, the authors offer concrete, practical tips as well as pertinent background information, together with insights into the optimization methods of seven major international companies from various sectors.
The whole is rounded off with real–life reports from experienced users coming from the different areas of application, in particular the life sciences, such as proteomics or drug development.

ESSENTIAL ASPECTS OF OPTIMIZATION
Principles of HPLC Optimization Illustrated by RP–Chromatography
Fast Gradient Separations
pH and Selectivity in Reversed–Phase Chromatography
Selecting the Correct pH–Value for HPLC
Optimization of Data Evaluation in Chromatography
Calibration Characteristics and Uncertainty indicating Starting–Points for Method Optimization
OPTIMIZATION IN DIFFERENT LC–MODI
Comparison and Choice of Columns for RP–HPLC
Column Selectivity in Reversed–Phase Chromatography
The Use of Principal Component Analysis for the Characterisation of Reversed–Phase Liquid Chromatographic Stationary Phases
Chemometrics –
A Powerful Tool for Handling a Large Number of Data
Linear Free Energy Relations –
Tools for Column Characterization and Method Optimization in HPLC?
Column Selectivity in Reversed–Phase Liquid Chromatography
Understanding Selectivity by Use of Suspended–State High Resolution Magic Angle Spinning NMR Spectroscopy
Optimization in Normal–Phase HPLC
Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection
Gel Filtration –
Size Exclusion Chromatography of biopolymers
Optimization in Affinity Chromatography
Optimization of Chiral Separations in HPLC
?LC/nano LC –
Optimization and Troubleshooting
Microchip–Based Liquid Chromatography –
Techniques and Possibilities
Ultra–Performance Liquid Chromatography
HYPHENATED TECHNIQUES
Immunochromatographic Techniques
Enhanced Characterization and Comprehensive Analyses by 2–dimensional Chromatography
LC/MS –
Hints and Recommendations for Optimization and Troubleshooting
LC–NMR–Coupling
COMPUTER–AIDED OPTIMIZATION
Computer–Facilitated HPLC Method Development Using DryLab Software
ChromSword Software for Automated and Computer–Assisted Development of HPLC Methods
Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC
USER REPORTS
NanoLC–MS/MS in Proteomics
Ways for the Verification of the Robustness in RP–HPLC
Separation of Complex Sample Mixtures
Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC–UV/MS/CLND

Following his PhD thesis in 1983, Stavros Kromidas worked as a sales manager for Waters Chromatography . From 1989 to 2001 he was Managing Director of Novia GmbH, a consultancy company for analytical chemistry, and he has been an independent consultant since then. He has been working in the field of HPLC since 1978 and has given lectures and training courses since 1984. He is the author and co–author of numerous articles and several successful books.

"A valuable text by any estimation, I would recommend it highly for hands–on users or managers directing a group of chromatographers toward more efficient and effective HPLC separations." ( Journal of the American Chemical Society, February 21, 2007)