RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals

This hand–on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids.
In easy to follow step–by–step protocols you will learnhow RNAi works in plants, flies and mammalshow to design the most efficient RNAi constructshow to achieve transient, stable and conditional RNAi in cell cultureshow to determine the efficiency of an RNAi experimenthow to use RNAi for gene therapyAll protocols have been thoroughly tested in the author′s laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments.
Includes a list of suppliers for RNAi reagents and equipment and a glossary of terms.

Spis treści:
Preface.
1 Introduction: RNA interference, the "Breakthrough of the Year 2002".
2 RNAi in Caenorhabditis elegans.
3 RNAi in Drosophila.
4 RNAi in Mammals.
Appendix 1: Abbreviations.
Appendix 2: List of Protocols.
Appendix 3: Suppliers of RNAi–related Chemicals and Probes.
Appendix 4: Glossary of Terms.
Subject Index.

Nota biograficzna:
Ute Schepers studied Chemistry at the Kekulé–Institut für Organische Chemie und Biochemie, University of Bonn (Germany), where she completed her PhD thesis in the laboratory of Prof. Dr. K. Sandhoff in 1997. She then moved to Boston for a post–doctoral fellowship in the laboratory of Tomas Kirchhausen at the Department of Cell Biology, Harvard Medical School. Since her return to Bonn in 2000 she is pursuing her own research projects using a combination of chemical and genetic methods for the further development of RNA interference in mammals. One of her main efforts is the chemical synthesis of novel molecular transporters for therapeutical RNAi application.

Okładka tylna :
This hand–on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids.
In easy to follow step–by–step protocols you will learnhow RNAi works in plants, flies and mammalshow to design the most efficient RNAi constructshow to achieve transient, stable and conditional RNAi in cell cultureshow to determine the efficiency of an RNAi experimenthow to use RNAi for gene therapyAll protocols have been thoroughly tested in the author′s laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments.
Includes a list of suppliers for RNAi reagents and equipment and a glossary of terms.