Molecular Imprinting: From Fundamentals to Applications

Molecular imprinting, the polymerization of monomers in the presence of a template molecule which imprints structural information into the resulting polymers, is a scientific field which is rapidly gaining significance for a widening range of applications in biotechnology, biochemistry and pharmaceutical research.
The methods and tools needed to distinguished target molecules from others by means of tailor–made receptors are constantly growing in importance and complexity. This book gives a concise and highly up–to–date overview of the remarkable progress made in this field in the last five years.
The material is comprehensively presented by the authors, giving a thorough insight into fundamentals and applications for researchers in both industry and academy.

Spis treści:
1. INTRODUCTION.
Importance of Receptor Molecules in Advanced Science and Technology.
Naturally Occurring Receptors.
Artificial Receptors.
Receptors for Practical Applications.
Why is the Molecular Imprinting Method so Promising?
2. FUNDAMENTALS OF MOLECULAR IMPRINTING.
Introduction.
General Principle of Molecular Imprinting.
Covalent Imprinting and Non–covalent Imprinting.
Advantages and Disadvantages of Covalent and Non–covalent Imprinting.
History.
3. EXPERIMENTAL METHODS (1) –
PROCEDURES OF MOLECULAR IMPRINTING.
Introduction.
Reagents and Experimental Procedures.
Covalent Imprinting.
Non–covalent Imprinting.
»Dummy Molecular Imprinting«.
4. EXPERIMENTAL METHODS (2) –
EVALUATION OF IMPRINTING EFFICIENCY.
Introduction.
Chromatographic Experiments.
Batchwise Guest–Binding Experiments.
Determination of Guest–Binding Constants.
5. SPECTROSCOPIC ANATOMY OF MOLECULAR IMPRINTING REACTIONS.
Introduction.
Structures of Adducts in the Pre–organization Step.
Determination of  Binding Constant K for the Formation of Monomer–Template Adduct.
Relationship between K Value and Imprinting Efficiency.
Structure of the Guest–Binding Site.
6. FLOW CHART OF A TYPICAL MOLECULAR IMPRINTING.
Introduction.
Choice of Agents.
Polymerization.
Packing of Imprinted Polymer into the HPLC Column.
Quantitative Evaluation of Imprinting Efficiency.
7. APPLICATIONS OF MOLECULARLY IMPRINTED POLYMERS.
Introduction.
Sensor Applications.
Signaling Polymers.
Molecularly Imprinted Sorbent Assays.
Molecularly Imprinted Membranes.
Affinity–based Solid–phase Extraction.
In Situ Preparation of Imprinted Polymers.
Molecularly Imprinted Catalysts.
8. RECENT CHALLENGES AND PROGRESS.
Introduction.
Molecular Imprinting in Water.
Use of Two Kinds of Functional Monomers for Cooperative Recognition.
Inorganic Gel as the Matrix for Molecular Imprinting.
Artificial Enzyme (Molecular Catalyst) by Molecular Imprinting.
Catalytic Antibody Prepared by Using Transition–State Analog.
9. CONCLUSIONS AND PROSPECTS.
Index.

Okładka tylna:
Molecular imprinting, the polymerization of monomers in the presence of a template molecule which imprints structural information into the resulting polymers, is a scientific field which is rapidly gaining significance for a widening range of applications in biotechnology, biochemistry and pharmaceutical research.
The methods and tools needed to distinguished target molecules from others by means of tailor–made receptors are constantly growing in importance and complexity. This book gives a concise and highly up–to–date overview of the remarkable progress made in this field in the last five years.
The material is comprehensively presented by the authors, giving a thorough insight into fundamentals and applications for researchers in both industry and academy.