Biochemical Methods: A Concise Guide for Students and Researchers

This book gives a profound overview on the relevant biochemical techniques. Moreover, it refers to laboratory equipment and safety aspects and explains how to obtain relevant biochemical information. It provides an introduction into physical–chemical processes and mathematical methods required for the interpretation of data. Principles of expensive instrumental analysis are also explained and a presentation of safety considerations and regulatory issues according to international requirements is given.
With its practical approach the book is not only highly useful for professionals – laboratory technicians and scientists – but also for students.
Special feature: a CD–ROM on quantitative analysis of biochemical experiments!
"... An ideal how–to for those working in biochemistry."
CHEMIE in unserer Zeit
"... and anyone working in a biochemical laboratory will find it useful. Strongly recommended."
Laboratory News

Spis treści:
PREFACE
BIOCHEMICAL LITERATURE
Accessing the biochemical literature
Access to the methodologically–based biochemical literature
Reference works and handbooks
Literature searches
Documentation of practical work
Literature
GENERAL LABORATORY PROCEDURES
The biochemistry laboratory
Routine biochemical procedures
Working with radioactivity
Literature
SAMPLE PREPARATION
Cell and tissue disruption
Solubilisation
Precipitation procedures for proteins and nucleic acids
Dialysis, ultrafiltration and lyophilisation
Literature
SEPARATION METHODS
Chromatography
Electrophoresis
Centrifugation
Literature
ANALYTICAL METHODS
Protein analysis
Nucleic acid analysis
Enzymatic analysis
Literature
IMMUNOLOGICAL METHODS
Antibodies
Antibody–antigen interactions
Literature
BIOPHYSICAL METHODS
Spectroscopy
Scattering techniques
Interactions
Determination of structure
Literature< br>MATHEMATICAL METHODS
Statistics
Quantitative evaluation of experimental results
Sequence analysis
Literature
QUANTITATIVE ANALYSIS OF BIOCHEMICAL DATA
Introduction
Applications
Guide to the compact disc
APPENDIX I: SI–UNITS
APPENDIX II: CONVERSIONS INTO SI–UNITS
INDEX
+ CD containing Chapter 9 and Exercises to Chapter 9