Human Pluripotent Stem Cells: A Practical Guide

Comprehensive coverage of the entire induced pluripotent stem cell basic work flow

Pluripotent stem cells (PSC) can divide indefinitely, self–renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder–dependent and feeder–free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self–renewal marker expression and differentiation potential.

Human Pluripotent Stem Cells: A Practical Guide offers in–depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more.

Includes helpful tips and tricks that are normally omitted from regular research papers Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations Presents specific sections (i.e., reprogramming, differentiation) in a concise and easily digestible manner Written by experts with extensive experience in stem cell technologies

Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

Table of Contents Aseptic Techniques  Mammalian Cell Culture Basics Review of Biochemical Calculations  Introduction to Stem Cells PSC Culture Reprogramming Differentiation PSC Characterization Other Considerations Appendix I: Reagents, Medias, and Solutions Appendix II: Common Antibodies Appendix III: Consumable and Equipment Appendix IV: Logs and Formas

Uma Lakshmipathy, Director R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Chad C. MacArthur, Staff Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Mahalakshmi Sridharan, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Rene H. Quintanilla, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA