Chromatography: Principles and Instrumentation

Provide students and practitioners with a solid grounding in the theory of chromatography and the metrics used in its applications

In this book, you will learn the fundamental principles underpinning chromatography and be able to connect those principles to the design and use of chromatographic systems.

Chromatography is the most widely used technique in modern analytical chemistry. Chromatography: Principles and Instrumentation, designed as a textbook, is focused on the current theory and the modern practice of chromatography.  It is intended for use in both quantitative analysis courses and instrumental methods courses.

Chromatography: Principles and Instrumentation features:

Numerous figures and problems that help the reader visualize these processes and identify the key variables in any given chromatography problem Introduction to the most recent developments in chromatography Case studies that illustrate the range of applications addressed by chromatography such as the analysis of performance–enhancing drugs in sports and the detection of deliberate contamination of food Problems embedded throughout the chapters as well as at the end of each chapter so that students can check their understanding before continuing on to new sections Clear and concise writing intended for undergraduate students and analysts just entering the field of chromatography.

The book, split into three chapters, covers Fundamentals of Chromatography, Gas Chromatography, and Liquid Chromatography. Chapter 1 provides an in–depth description of the processes governing chromatography and includes a systematic development of band broadening. Chapter 2 focuses on gas chromatography (GC). It introduces the components of GC instruments in the context of the function they accomplish and discusses the theory behind instrumentation, instrument design, and practical aspects. The final chapter addresses liquid chromatography. It emphasizes reversed–phase liquid chromatography (RPLC) because of its prominence, but you will also find descriptions of all of the common modes of LC.

The text of each section includes numerous headings and subheadings, making it easy for faculty and students to refer to and use the information within each chapter selectively. Students in analytical chemistry and biochemistry courses focused on chromatography will find this book a valuable resource in providing them with the knowledge needed to operate in modern laboratories.

Mark F. Vitha is currently a Professor at Drake University.  He received his Ph.D. from the University of Minnesota.  He is the series editor for The Chemical Analysis Series (Wiley) and is a co–editor of the book High Throughput Analysis for Food Safety (Wiley, 2014). He has been named as a Levitt Teacher of the Year, a Windsor Professor of Science, and a Ronald D. Troyer Research Fellow at Drake.

 

Preface

Chapter 1:  Fundamentals of Chromatography

1.1 Theory

1.2 Band Broadening

1.3 General Resolution Equation

1.4 Peak symmetry

1.5 Key Operating Variables

1.6 Instrumentation

1.7 Practice of the Technique

1.8 Emerging Trends and Applications

1.9 Summary

Problems

Bibliography

References

Chapter 2:  Gas Chromatography

2.1 Theory of Gas Chromatographic Separations

2.2 Key Operating Variables that Control Retention

2.3 Gas Chromatography Instrumentation

2.4 A More Detailed Look at Stationary Phase Chemistry: Kovats Indices and McReynolds Constants

2.5 Gas Chromatography in Practice

2.6 A Real–World Application of Gas Chromatography

2.7 Summary

Problems

Bibliography

References

Chapter 3:  Liquid Chromatography

3.1 Examples of Liquid Chromatography Analyses

3.2 Scope of liquid chromatography

3.3 History of LC

3.4 Modes of Chromatography

3.5 HPLC Instrumentation

3.6 Specific Uses and Advances/Trends in Chromatography

3.7 Application of LC –– Analysis of Pharmaceutical Compounds in Groundwater

3.8 Summary

Problems

References

Index