MALDI–TOF and Tandem MS for Clinical Microbiology

This comprehensive handbook highlights the triumph and developments of MALDI–TOF mass spectrometry in diagnostic approaches to clinical microbiology over the past decade, and provides insight into new technologies including tandem MS/MS. Presenting the reader with detailed coverage of  current research and future opportunities, this volume, divided into two parts, with 21 chapters, includes such topics as:

          Applications of MALDI–TOF Mass Spectrometry in Clinical Diagnostic Microbiology

          The Potential of MALDI–TOF MS to Distinguish between Taxonomically Similar Species that Manifest Different Infections

          Microbial DNA Analysis by MALDI–TOF Mass Spectrometry

          Development and Application of MALDI–TOF MS for Detection of Resistance Mechanisms                                                                                                                              

          Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

          Top–Down Proteomics in the Study of Microbial Pathogenicity

          Proteogenomics of Non–Model Microorganisms

Primarily aimed at clinical microbiologists, diagnostic technologists, biomedical scientists, researchers, clinicians, academics and scientists in bio–resource centres, this book is also relevant to researchers, post–graduate scientists, technologists in food industry, pharmaceuticals, agriculture.

Preface

MALDI–TOF Mass Spectrometry

1. MALDI–TOF and LC–MS/MS: A Paradigm Shift from Research to Frontline Microbial Diagnostics; A Laboratory′s Vision and Relentless Resolve to Help Develop and Implement this New Technology Amidst Formidable Obstacles

2. Criteria for Development of MALDI–TOF Mass Spectral Database

3. Applications of MALDI–TOF Mass Spectrometry in Clinical Diagnostic Microbiology

4. The Challenges of Identifying Mycobacterium to the Species Level using MALDI–TOF MS

Section A: Modifications of Standard Bruker Biotyper Method

Section B: ASTA s MicroID System and its MycoMp Database for Mycobacteria

5. Transformation of Anaerobic Microbiology since the Arrival of MALDI–TOF–MS

6. The Potential of MALDI–TOF MS to Distinguish between Taxonomically Similar Species that Manifest Different Infections

7. Identification of Species in Mixed Bacterial Populations using MALDI–TOF MS

8. Microbial DNA Analysis byMALDI–TOF Mass Spectrometry

Section A:DNA Analysis of Viral Genomes using MALDI–TOF MS

Section B:Mass Spectral Analysis of Proteins of Nonculture and Cultured Viruses

9. Analysis of Yeast and Fungi using a Non–Commercial Database

10. Development and Application of MALDI–TOF MS for Detection of Resistance Mechanisms

11. Discrimination of Burkholderia, Brucellaspp and other Taxa at the Subspecies Level Using MALDI–TOF Mass Spectrometry

12. MALDI–TOF–MS Based Upon the Ribosomal Proteins Coding in S10 for Proteotyping

Tandem MS/MS based Approaches to Microbial Characterisation

13. Tandem Mass Spectrometry Analysis as an Approach to Delineate Genetically Related Taxa

14. Mapping of the Proteogenome of Clostridium difficile Isolates of Varying Virulence

15. Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

16. Proteotyping: Tandem Mass Spectrometry and Proteomic Analysis of Pathogenic Microorganisms

17. Proteogenomics of Pseudomonas aeruginosa in Chronic Cystic Fibrosis Infections

18. Top–Down Proteomics in the Study of Microbial Pathogenicity

19. Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions

20. Proteogenomics of Non–Model Microorganisms

21. Analysis of Mass Spectral Data for Species Identification and Typing of Microorganisms

Section A:The analysis of MALDI–TOF MS spectra using the BioNumerics software

Section B:Subtyping of Staphylococcus spp. based upon MALDI–TOF MS Data

Section C: Elucidating the Intra–Species Proteotypes of Pseudomonas aeruginosa from Cystic Fibrosis

Haroun N. Shah                   Department of Natural Sciences, Middlesex University, London, UK

Saheer E. Gharbia              Genomic Research, Public Health England, London, UK