Bioanalytical Chemistry

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry

The field of bioanalytical chemistry overlaps a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics all of which makes a comprehensive introduction to the subject even more essential for students and researchers. The Second Edition of Bioanalytical Chemistry follows the same path of the first edition by providing a thorough introduction to bioanalytical chemistry for students and practitioners. In so doing, it brings together many of the techniques commonly used by biochemists and molecular biologists.

Along with many other topics, the text includes entire chapters on design and implementation of enzyme assays; mass spectrometry; and validation of new methods. Each chapter progresses from basic concepts to applications involving real samples, and ends with a set of problems, while an appendix contains selected answers. The significantly expanded new edition includes three new chapters and focuses on the more recent developments in bioanalytical chemistry while minimizing some of the techniques that are less relevant. The authors have limited mathematical derivations to those that are essential for a basic understanding of the principles underlying each method.

Featuring many of the techniques used by biochemists and molecular biologists, Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples.

    Bioanalytical Chemistry covers:

    Biomimetic materials

    Lab–on–chip devices

    Spectroscopic methods for total protein, nucleic acids and carbohydrate

    Structural and functional properties of antibodies

    Immunoassays for antigen or antibody quantitation

    Principles and proteomics applications of 1D and 2D electrophoresis

    Centrifugation methods

    Mass spectrometry of biomolecules

    Quantitation of enzymes and their substrates

    Design and implementation of high–throughput enzyme assays

    Analytical methods

    Validation of new methods

    And much more

This indispensable guide features end–of–chapter problems and selected answers in the appendix, designed to help students assess their knowledge of each topic. Each chapter also includes suggestions for further reading.

More than any other reference, Bioanalytical Chemistry captures the interdisciplinary scope of this emerging field and uniquely prepares students for collaborative scientific work and research in industrial, government, and academic laboratories.

Susan R. Mikkelsen, PhD, is a Professor in the Department of Chemistry at the University of Waterloo, Ontario, Canada. She has presented at numerous conferences and is the author of over 35 peer–reviewed research articles and 115 presentations. She has organized and supervised a wide variety of bioanalytical research projects and has participated in local and international collaborations in this field. She is the co–author of the first edition of Bioanalytical Chemistry.

Eduardo Cortón, PhD, is Head of the Bioanálisis and Biosensors Laboratory in the Biochemistry Department at the University of Buenos Aires, Argentina.  He is also an Adjunct Professor in the Department of Biological Chemistry at the University of Buenos Aires as well as an active Adjunct Researcher at the National Council of Scientific and Technical Research (CONICET). He has published over 35 peer–reviewed research articles and presented at 80 conferences. He is the co–author of the first edition of Bioanalytical Chemistry.