Cell and Molecular Biology and Imaging of Stem Cells

Cell and Molecular Biology and Imaging of Stem Cells features original and review articles written by experts who have made significant contributions to stem cell biology and imaging. Chapters cover a broad spectrum of aspects of the field, including Stem cells from the Amniotic Fluid and Placenta, Biomaterials as Artificial Niches for Pluripotent Stem Cell Engineering, Low–Intensity Ultrasound in Stem Cells and Tissue Engineering, Mammalian Neo–Oogenesis from Ovarian Stem Cells, Oct4–EGFP Transgenic Pigs as a New Tool for Visualization of Pluripotent and Reprogrammed Cells, Regulation of Adult Intestinal Stem Cells, Stem Cell Therapy for Veterinary Orthopedic Lesions, Sex Steroid Combinations in Regenerative Medicine for Brain and Heart Diseases, Hair Follicle Stem Cells, The Potential of Using Induced Pluripotent Stem Cells in Skin Diseases, Mitochondrial Differentiation in Early Embryo Cells and Pluripotent Stem Cells, and The Role of Centrosomes in Cancer Stem Cell Functions. Cell and Molecular Biology and Imaging of Stem Cells addresses a wide variety of cell and molecular topics in unprecedented detail, and is a must–read for graduate students and academic and industry professionals in the expanding field of stem cell biology. Reviews new imaging tools and markers for cell and molecular imaging in stem cell biology. Covers novel aspects of stem cell imaging in reproductive biology and stem cell niches Includes chapters on the developing area of centrosome biology as it applies to embryonic and adult stem differentiation

Contributors vii Preface xi 1 Cell and Molecular Biology and Imaging of Stem Cells: Stem Cells from the Amniotic Fluid and Placenta 1 Amritha Kidiyoor, Sean V. Murphy, and Anthony Atala 2 Biomaterials as Artificial Niches for Pluripotent Stem Cell Engineering 21 Kyung Min Park and Sharon Gerecht 3 Low–Intensity Ultrasound in Stem Cells and Tissue Engineering 45 Byung Hyune Choi, Kil Hwan Kim, Mrigendra Bir Karmacharya, Byoung–Hyun Min and So Ra Park 4 Mammalian Neo–Oogenesis from Ovarian Stem Cells In Vivo and In Vitro 67 Antonin Bukovsky and Michael R. Caudle 5 Oct4–EGFP Transgenic Pigs as a New Tool for Visualization of Pluripotent and Reprogrammed Cells 137 Monika Nowak–Imialek and Heiner Niemann 6 Regulation of Adult Intestinal Stem Cells through Thyroid Hormone–Induced Tissue Interactions during Amphibian Metamorphosis 153 Atsuko Ishizuya–Oka 7 Stem Cell Therapy for Veterinary Orthopedic Lesions 173 Anna Paula Balesdent Barreira and Ana Liz Garcia Alves 8 Sex Steroid Combinations in Regenerative Medicine for Brain and Heart Diseases: The Vascular Stem Cell Niche and a Clinical Proposal 193 Antonin Bukovsky and Michael R. Caudle 9 Hair Follicle Stem Cells 211 Hilda Amalia Pasolli 10 The Potential of Using Induced Pluripotent Stem Cells in Skin Diseases 223 Shigeki Ohta, Ophelia Veraitch, Hideyuki Okano, Manabu Ohyama, and Yutaka Kawakami 11 Mitochondrial Differentiation in Early Embryo Cells and Pluripotent Stem Cells 247 Heide Schatten, Qing–Yuan Sun, and Randall S. Prather 12 The Role of Centrosomes in Cancer Stem Cell Functions 259 Heide Schatten Index 281

Heide Schatten is Professor in the Department of Veterinary Pathobiology at the University of Missouri