Functional Imaging by Controlled Nonlinear Optical Phenomena

A comprehensive guide to theories, techniques, and technologies for the functional imaging of biological systems using nonlinear microscopy Nonlinear optical imaging using ultrafast laser pulses is a powerful microscopy technique that has become a valued research tool within the biological sciences. Allowing for very high-precision imaging and manipulation of cells, nonlinear optical imaging is revolutionizing our understanding of functional processes in living tissue, and the number of research and clinical applications for it continues to grow. Written by a team of distinguished experts from the University of Osaka, this is a comprehensive guide to state-of-the-art nonlinear microscopy of biological systems using ultrafast laser pulses. The authors review the evolution of nonlinear optical microscopy, from its origins in the 1990s to today's most advanced photo-controlled techniques, and explore an array of vital topics, including: Conventional nonlinear optical microscopy Ultrafast laser optics required for nonlinear optical microscopy Ultrafast laser manipulation in biological and biomedical research Functional imaging based on molecular control The full range of current nonlinear optical microscopic techniques, from two-photon absorption microscopy to nonresonant four-wave mixing microscopy An array of biological applications, including nanosurgery and transfection Providing balanced coverage of both theory and practice, Functional Imaging by Controlled Nonlinear Optical Phenomena is an indispensable primer for cell biologists, life scientists, and biomedical researchers, engineers, and technicians.

KEISUKE ISOBE, DEng, is a Research Scientist at the RIKEN Center for Advanced Photonics. WATARU WATANABE, DEng, is a Professor in the Department of Electrical & Electronic Engineering, College of Science and Engineering, at Ritsumeikan University. KAZUYOSHI ITOH, DEng, is a Professor Emeritus and Research Professor at the Science Technology Entrepreneurship Laboratory (E-Square), Osaka University.