Genetic Manipulation: Techniques and Applications

Over the last few years the life sciences have been revolutionized by the introduction of techniques for the analysis and cloning of DNA, the insertion of natural or synthesized genetic material into alternative host cells and the isolation of gene products from modified cells. These procedures are collectively termed genetic manipulation or, more popularly, genetic engineering. Genetic manipulation is no longer the province of the specialised researcher. The techniques are now finding widespread application in all fields of medicine and biology and the materials required are increasingly available commercially. Nevertheless, application of available techniques to new areas of

Spis treści:
Extraction, purification and assay of DNA; Restriction mapping of DNA; The construction and use of cloning vectors; Adaptor based cDNA cloning by the phage vectors; Plasmid profiling and DNA/DNA hybridization for distinguishing between mesophilic aeromonas bacteria; Preparation and screening of genomic libraries; DNA probes for detection and identification of bacteria; Extraction and purification of eukaryotic mRNA; cDNA cloning; Primer extension sequencing of RNA viruses; Forensic applications of DNA profiling; Application of nucleic acid probes to the identification of bacterial enteric pathogens; Quantification of collagen mRNA levels in mammalian cells; Detection of food–borne bacterial pathogens by a colorimetric DNA hybridization method; Use of a DNA probe for a viroid in plant pathology; Analysis of restriction fragment length polymorphisms in the study of bacteria; The use of DNA probes to detect `selected bacteria′ after their introduction into the waste water treatment plant; The use of genetic manipulation to study bacterial pathogens; RNA detection methods in basic cardiac research; The polymerase chain reaction and its application to basic research in molecular biology; The effect of nutrient limitation on the stability and expressio n of recombinant plasmids; Large scale production of proteins from recombinant DNA in Escherichia coli; Stability and copy number of yeast cloning vectors; Designer genes – Design and synthesis illustrated for the gene coding for human macrophage colony stimulating factor; Monitoring safety in process biotechnology; Patenting in biotechnology

Okładka tylna:
Over the last few years the life sciences have been revolutionized by the introduction of techniques for the analysis and cloning of DNA, the insertion of natural or synthesized genetic material into alternative host cells and the isolation of gene products from modified cells. These procedures are collectively termed genetic manipulation or, more popularly, genetic engineering. Genetic manipulation is no longer the province of the specialised researcher. The techniques are now finding widespread application in all fields of medicine and biology and the materials required are increasingly available commercially. Nevertheless, application of available techniques to new areas of