Chemical Genomics

Advances in chemistry, biology and genomics coupled with laboratory automation and computational technologies have led to the rapid emergence of the multidisciplinary field of chemical genomics. This edited text, with contributions from experts in the field, discusses the new techniques and applications that help further the study of chemical genomics. The beginning chapters provide an overview of the basic principles of chemical biology and chemical genomics. This is followed by a technical section that describes the sources of small-molecule chemicals; the basics of high-throughput screening technologies; and various bioassays for biochemical-, cellular- and organism-based screens. The final chapters connect the chemical genomics field with personalized medicine and the druggable genome for future discovery of new therapeutics. This book will be valuable to researchers, professionals and graduate students in many fields, including biology, biomedicine and chemistry.

Spis treści:
Part I. Overviews
1. Harnessing the power of chemistry for biology and medicine Cheryl L. Meyerkord and Haian Fu
2. Chemical genomics, exploring biology with small organic molecules Nicholas Aberle and Craig M. Crews
3. Chemical proteomics Akihisa Matsuyama, Yoko Yashiroda and Minoru Yoshida
Part II. Molecules for Chemical Genomics
4. Diversity-oriented synthesis Warren R. J. D. Galloway, Richard J. Spandl, Andreas Bender, Gemma L. Thomas, Monica Diaz-Gavilan, Kieron M. G. OConnell and David R. Spring
5. Targeted chemical libraries Gregory P. Tochtrop and Ryan E. Looper
6. Fragment-based ligand discovery Sandra Bartoli, Antonella Squarcia and Daniela Fattori
7. Basics and principles for building natural product-based libraries for HTS Ronald J. Quinn
Part III. Basics of High Throughput Screening
8. Essentials for high throughput screening operations Stewart P. Rudnicki, James V. Follen, Nicola J. Tolliday and Caroline E. Shamu
9. High content analysis and screening, basics, instrumentation and applications Paul A. Johnston
10. Phenotypic screens with model organisms Grant N. Wheeler, Robert A. Field and Matthew L. Tomlinson
11. Screening informatics and ChemInformatics Lestyn Lewis
Part IV. Chemical Genomics Assays and Screens
12. Basics on HTS assay design and optimization Eduard Sergienko
13. Molecular sensors for transcriptional and post-transcriptional assays Douglas S. Auld and Natasha Thorne
14. Fluorescence-based platforms for HTS assays – FP and TR-FRET Yuhong Du and Jonathan Havel
15. Compound profiling with high content screening methodology Thomas Mayer and Stephan Schürer
16. Use of transgenic zebrafish in a phenotypic screen for angiogenesis inhibitors Jaeki Min, Yuhong Du, Brenda Bondesen, Brian Revennaugh, Peter Eimon and Ray Dingledine
17. Flow cytometry multiplexed screening methodologies Virginia M. Salas, J. Jacob Strouse, Zurab Surviladze, Irena Ivnitski-Steele, Bruce S. Edwards and Larry A. Sklar
18. Basic principles and practices of computer-aided screening and drug design Chao-Yie Yang, Denzil Bernard and Shaomeng Wang
19. Computational approach for drug target identification Honglin Li, Mingyue Zheng, Xiaofeng Liu and Hualiang Jiang
20. Label-free biosensor technologies for assay development and HTS Yuhong Du and Arron Xu
Part V. Chemical Genomics and Medicine
21. Pharmacogenomics to link genetic background with therapeutic efficacy and safety Mark M. Bouzyk, Weining Tang and Brian Leyland-Jones
22. Drugs, genomic response signatures, and customized cancer therapy Rafael Rosell, Teresa Moran and Miguel Taron
Appendix. Current drug targets and the druggable genome Andreas Russ.