Cytometric Analysis of Cell Phenotype and Function

Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings, following improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding and apoptosis. Guidance is given on data interpretation, quality control procedures, pitfalls and problems. All chapters contain detailed protocols from leading authorities in the field with extensive practical experience.

Spis treści:
1. Principles of flow cytometry Marion G. Macey
2. Introduction to the general principles of sample preparation Desmond A. McCarthy
3. Fluorescence and fluorochromes Desmond A. McCarthy
4. Quality control in flow cytometry David Barnett and John T. Reilly
5. Data analysis in flow cytometry Mark W. Lowdell
6. Laser scanning cytometry, application to the immunophenotyping of hematological malignancies Richard J. Clatch
7. Leucocyte immunobiology Marion G. Macey and Timothy M. Milne
8. Immunophenotypic analysis of leucocytes in disease Jamie D. Cavenagh and Marion G. Macey
9. Analysis and isolation of minor cell populations Terry Hoy
10. Cell cycle, DNA and DNA ploidy analysis Paul D. Allen and Adrian C. Newland
11. Cell viability, necrosis and apoptosis Paul D. Allen and Adrian C. Newland
12. Phagocyte biology and function Marion G. Macey
13. Intracellular measures of signalling pathways James W. Jacobberger and David W. Hedley
14. Cell-cell interactions Marion G. Macey and Mary R. Cahill
15. Nucleic acids Charles L. Goolsby
16. Microbial infections David Lloyd, David J. Mason and Marc T. E. Suller
17. Leucocyte cell surface antigens Marion G. Macey
18. Recent and future developments, conclusions Desmond A. McCarthy
Appendix 1. Abbreviations
Appendix 2. Useful Internet sites.