ELISA and Other Solid Phase Immunoassays: Theoretical and Practical Aspects

ELISA and Other Solid Phase Immunoassays Theoretical and Practical Aspects D. M. Kemeny, Department of Medicine, and S. J. Challacombe, Department of Oral Medicine and Pathology, United Medical and Dental Schools of Guy’s and St Thomas’s Hospitals, London, UK This guide to the modern applications of solid phase immunoassays is written by experts from a wide range of specialities who have used ELISA and other assays in a variety of fields. Not only is the book ideally suited for use as a laboratory manual but, recognizing the importance of understanding the theoretical basis of such assays, the authors also provide a comprehensive account of the scientific principles involved. The book analyses the solid phase supports used, as well as amplification systems, quantification and affinity of antibodies, detection systems, and the application of assays to cell biology, immunology and microbiology. Laboratory workers in the fields of medicine, dentistry, biochemistry, immunology, food science, and forensic and veterinary medicine will find this an invaluable source of advice, guidance and information on solid phase immunoassays. Other titles of interest from Wiley include two books edited by Professor W. P. Collins—Alternative Immunoassays and Complementary Immunoassays—as well as the following: Allergy: An International Textbook Edited by M. H. Lessof, T. H. Lee and D. M. Kemeny

Spis treści:
BASIC ASPECTS.
An Introduction to ELISA (D. Kemeny & S. Chanter).
Microtitre Plates and Other Solid Phase Supports (D. Kemeny & S. Challacombe).
Quantitative Aspects of Solid Phase Immunoassays (R. Hamilton & N. Atkinson).
AMPLIFICATION.
Amplification by Second Enzymes (A. Johannsson & D. Bates).
The Amplified ELISA (a–ELISA): Immunochemistry and Applications (J. Butler).
MOLECULAR ASPECTS.
The Role of Antibody Affinity in the Performance of Solid Phase Assays (M. Devey & M. Steward).
The Immunochemistry of Sandwich ELISAs: Principles and Applications for the Quantitative Determination of Immunoglobulins (J. Butler).
The Use of ELISA in the Characterization of Protein Antigen Structure and Immune Response (A. Pesce & J. Michael).
The Modified Sandwich ELISA (SELISA) for the Detection of IgE and Other Antibody Isotypes (D. Kemeny).
CELLULAR ELISAs.
The Solid Phase Enzyme–Linked Immunospot Assay (ELISPOT) for Enumerating Antibody–Secreting Cells: Methodology and Applications (C. Czerkinsky, et al.).
ELISA–Plaque Assay for the Detection of Single Antibody–Secreting Cells (J. Sedgwick & P. Holt).
CHEMILUMINESCENCE.
Chemiluminescence Immunoassay (I. Weeks & J. Woodhead).
APPLICATIONS TO MICROBIOLOGY.
The Use of ELISA for Rapid Viral Diagnosis: Viral Antigen Detection in Clinical Specimens (S. Chantler & A. Clayton).
The Use of ELISA for Rapid Viral Diagnosis: Antibody Detection (R. Duncan).
Application of ELISA to Bacteriology (S. Challacombe).
Appendix.
Index.

Okładka tylna:
ELISA and Other Solid Phase Immunoassays Theoretical and Practical Aspects D. M. Kemeny, Department of Medicine, and S. J. Challacombe, Department of Oral Medicine and Pathology, United Medical and Dental Schools of Guy’s and St Thomas’s Hospitals, London, UK This guide to the modern applications of solid phase immunoassays is written by experts from a wide range of specialities who have used ELISA and other assays in a variety of fields. Not only is the book ideally suited for use as a laboratory manual but, recognizing the importance of understanding the theoretical basis of such assays, the authors also provide a comprehensive account of the scientific principles involved. The book analyses the solid phase supports used, as well as amplification systems, quantification and affinity of antibodies, detection systems, and the application of assays to cell biology, immunolog y and microbiology. Laboratory workers in the fields of medicine, dentistry, biochemistry, immunology, food science, and forensic and veterinary medicine will find this an invaluable source of advice, guidance and information on solid phase immunoassays. Other titles of interest from Wiley include two books edited by Professor W. P. Collins—Alternative Immunoassays and Complementary Immunoassays—as well as the following: Allergy: An International Textbook Edited by M. H. Lessof, T. H. Lee and D. M. Kemeny