Measuring in vivo Oxidative Damage: A Practical Approach

Fifteen years ago the field of oxygen free radicals was just beginning to launch into a new area of importance in pathology. Since then, oxygen free radicals have been implicated in a number of disease processes, including atherosclerosis and chronic inflammation. Measuring in vivo Oxidative Damage brings together methods by leading experts in the field of oxidative damage and by scientists from clinical biochemistry laboratories who have had much experience with the practical problems of measuring oxidative damage in vivo. Many of the authors are involved in national and international quality assurance programmes and routinely establish these assays in clinical research laboratories. The book is divided into 5 parts:
∗ Chromatographic procedures
∗ Measurement of 8–oxo deoxyguanosine
∗ Cellular–based methods
∗ Molecular–based assays
∗ Antioxidant activity.
Each part is designed to help the clinical scientist evaluate the best method for their particular problem in measuring oxidative damage in vivo. The methods represented are the ones most commonly used and are deemed robust and simple enough to apply to clinical material. Measuring in vivo Oxidative Damage is an ideal practical reference work for the clinical scientist and is a must for all laboratories in hospitals and research institutions which are actively involved in analytical work.

Spis treści:
CHROMATOGRAPHIC PROCEDURES.
Lipid Peroxidation Determination by HPLC (R. Bevan).
Measurement of Oxidized Bases in DNA and Biological Fluids by Gas Chromatography Coupled to Mass Spectrometry (T. Douki, et al.).
The Measurement of Protein Oxidation by HPLC (H. Griffiths, et al.).
MEASUREMENT OF 8–OXO DEOXYGUANOSINE.
Measurement of 8–Oxo–2′ –deoxyguanosine in Cellular DNA by High Performance Liquid Chromatography–Electrochemical Detection (M. Evans).
Immunochemical Detection of 8–Oxodeoxyguanosine in DNA (M. Cooke & K. Herbert).
Urinary Measurement of 8–OxodG (8–Oxo–2′ –deoxyguanosine) (H. Poulsen, et al.).
CELLULAR–BASED METHODS.
Measurement of Oxidative DNA Damage Using the Comet Assay (A. Collins).
Measuring Oxidative DNA Damage by Alkaline Elution (M. Pflaum & B. Epe).
MOLECULAR–BASED ASSAYS.
A ¯32P–Postlabelling Protocol to Measure Oxidative DNA Damage (G. Jones & M. Weinfeld).
Mapping Reactive Oxygen–Induced DNA Damage at Nucleotide Resolution (H. Rodriguez & S. Akman).
ANTIOXIDANT ACTIVITY.
Measurements of Plasma Antioxidant Activity (S. Maxwell).
Index.

Okładka tylna:
Fifteen years ago the field of oxygen free radicals was just beginning to launch into a new area of importance in pathology. Since then, oxygen free radicals have been implicated in a number of disease processes, including atherosclerosis and chronic inflammation. Measuring in vivo Oxidative Damage brings together methods by leading experts in the field of oxidative damage and by scientists from clinical biochemistry laboratories who have had much experience with the practical problems of measuring oxidative damage in vivo. Many of the authors are involved in national and international quality assurance programmes and routinely establish these assays in clinical research laboratories. The book is divided into 5 parts:
∗ Chromatographic procedures
∗ Measurement of 8–oxo deoxyguanosine
∗ Cellular–based methods
∗ Molecular–based assays
∗ Antioxidant activity.
Each part is designed to help the clinical scientist evaluate the best method for their particular problem in measuring oxidative damage in vivo. The methods represented are the ones most commonly used and are deemed robust and simple enough to apply to clinical material. Measuring in vivo Oxidative Damage is an ideal p ractical reference work for the clinical scientist and is a must for all laboratories in hospitals and research institutions which are actively involved in analytical work.