Culture of Hematopoietic Cells

Provides scientists with step–by–step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.

Spis treści:
Partial table of contents:
PRIMITIVE PROGENITOR ASSAYS.
Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher).
Assay for Murine Blast Cell Colonies (N. Katayama & M. Ogawa).
Serum–Deprived Cultures of Primary Hematopoietic Cells (G. Migliaccio & A. Migliaccio).
COLONY–FORMING ASSAYS FOR MALIGNANT CELLS.
Colony–Forming Assays for Acute Myeloid Leukemia (S. Rogers, et al.).
LONG–TERM ASSAYS AND IMMORTALIZATION.
Long–Term Culture of Human Myeloid Cells (H. Sutherland & C. Eaves).
Immortalization of Hematopoietic Cells (C. MacDonald).
PURIFICATION OF PROGENITOR CELLS.
Purification of Murine Granulocyte–Macrophage Progenitor Cells (CFU–GM) Using Counterflow Centrifugal Elutriation (S. Cooper & H. Broxmeyer).
OTHER SYSTEMS AND ASSAYS.
Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins & R. Fleischman).
List of Suppliers.
Index.