Bioanalytical Chemistry

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry
Bioanalytical chemistry is playing an increasingly central role in the fields of academic and industrial science. It overlaps with a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics–all of which makes a comprehensive introduction to the subject even more essential for students and researchers.
Featuring many of the techniques used by biochemists and molecular biologists, Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. The end result is an authoritative primer to this expanding field–one that will prove invaluable for advanced undergraduate chemistry and biochemistry students, bioanalytical chemistry graduate students, and practitioners. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples.Bioanalytical Chemistry covers:Spectroscopic methods for matrix characterizationStructural and functional properties of antibodiesPrinciples of electrophoresisCentrifugation methodsMass spectrometry of biomoleculesQuantitation of enzymes and their substratesDesign and implementation of enzyme assaysIsoelectric focussingChromatography of biomoleculesValidation of new bioanalytical methodsAnd much more
This indispensable guide features end–of–chapter problems and selected answers in the appendix, designed to help students assess their knowledge of each topic. Each chapter also includes suggestions for further reading, and mathematical derivations have been kept to a minimum throughout.
More than any other reference, Bioanalytical Chemistry captures the interdisciplinary scope of this emerging field–and uniquely prepares students for collaborative scientific work and research in industrial, government, and academic laboratories.

Spis treści:
Preface.
Acknowledgments.
1. Spectroscopic Methods for Matrix Characterization.
2. Enzymes.
3. Quantitation of Enzymes and Their Substrates.
4. Immobilized Enzymes.
5. Antibodies.
6. Quantitative Immunoassays with Labels.
7. Biosensors.
8. Directed Evolution for the Design of Macromolecular Bioassay Reagents.
9. Principles of Electrophoresis.
10. Applications of Zone Electrophoresis.
11. Isoelectric Focusing.
12. Capillary Electrophoresis.
13. Centrifugation Methods.
14. Chromatography of Biomolecules.
15. Mass Spectrometry of Biomolecules.
16. Validation of New Bioanalytical Methods.
Suggested References.
References.
Answers to Problems.
Index.

Nota biograficzna:
Susan R. Mikkelsen, PhD, is a professor in the Department of Chemistry at the University of Waterloo, Ontario, Canada.
Eduardo Cortón, PhD, is an adjunct professor in the Department of Biological Chemistry at the University of Buenos Aires, Argentina. He is also an investigator for the National Council of Scientific and Technical Research (CONICET) in Argentina.

Okładka tylna:
A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry
Bioanalytical chemistry is playing an increasingly central role in the fields of academic and industrial science. It overlaps with a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics–all of which makes a comprehensive introduction to the subject even more essential for students and researchers.
Featuring many of the techniques used by biochemists and molecular biologists, Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. The end result is an authoritative primer to this expanding field–one that will prove invaluable for advanced unde rgraduate chemistry and biochemistry students, bioanalytical chemistry graduate students, and practitioners. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples.Bioanalytical Chemistry covers:Spectroscopic methods for matrix characterizationStructural and functional properties of antibodiesPrinciples of electrophoresisCentrifugation methodsMass spectrometry of biomoleculesQuantitation of enzymes and their substratesDesign and implementation of enzyme assaysIsoelectric focussingChromatography of biomoleculesValidation of new bioanalytical methodsAnd much more
This indispensable guide features end–of–chapter problems and selected answers in the appendix, designed to help students assess their knowledge of each topic. Each chapter also includes suggestions for further reading, and mathematical derivations have been kept to a minimum throughout.
More than any other reference, Bioanalytical Chemistry captures the interdisciplinary scope of this emerging field–and uniquely prepares students for collaborative scientific work and research in industrial, government, and academic laboratories.