Force Microscopy: Applications in Biology and Medicine

A complete examination of the uses of the atomic force microscope in biology and medicine
This cutting–edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function.
The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X–ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore—the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers:Identification of the "porosome" in the growth hormone secreting cell of the pituitary glandProbing the structural and physical properties of microbial cell surfacesScanning probe microscopic characterization of the higher plant cell wall and its componentsCase studies of nano drug delivery systems using engineered dendrimersAFM techniques for studying living cellsInvestigating the intermolecular forces of leukocyte adhesion moleculesProtein–protein interactionsMicromechanical properties of lipid bilayers and vesicles
The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine.
This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it pr ovide the theory, but also practical considerations such as the selection of the right tools and approach.

Spis treści:
Preface.
Contributors.
Chapter 1. Porosome: The Universal Secretory Machinery in Cells (Bhanu P. Jena).
Chapter 2. Molecular Mechanism of SNARE–Induced Membrane Fusion (Bhanu P. Jena).
Chapter 3. Molecular Mechanism of Secretory Vesicle Content Expulsion During Cell Secretion (Bhanu P. Jena).
Chapter 4. Fusion Pores in Growth–Hormone–Secreting Cells of the Pituitary Gland: An AFM Study (Lloyd L. Anderson and Bhanu P. Jena).
Chapter 5. Properties of Microbial Cell Surfaces Examined by Atomic Force Microscopy (Yves F. Dufre&ncirc;e).
Chapter 6. Scanning Probe Microscopy of Plant Cell Wall and Its Constituents (Ksenija Radotić, Miodrag Mićić, and Milorad Jeremić).
Chapter 7. Cellular Interactions of Nano Drug Delivery Systems (Rangaramanujam M. Kannan, Omathanu Pillai Perumal, and Sujatha Kannan).
Chapter 8. Adapting AFM Techniques for Studies on Living Cells (J. K. Heinrich Hörber)
Chapter 9. Intermolecular Forces of Leukocyte Adhesion Molecules (Xiaohui Zhang and Vincent T. Moy).
Chapter 10. Mechanisms of Avidity Modulation in Leukocyte Adhesion Studied by AFM (Ewa P. Wojcikiewicz and Vincent T. Moy).
Chapter 11. Resolving the Thickness and Micromechanical Properties of Lipid Bilayers and Vesicles Using AFM (Guangzhao Mao and Xuemei Liang).
Chapter 12. Imaging Soft Surfaces by SFM (Andreas Janke and Tilo Pompe).
Chapter 13. High–Speed Atomic Force Microscopy of Biomolecules in Motion (Tilman E. Schäffer).
Chapter 14. Atomic Force Microscopy in Cytogenetics (S. Thalhammer and W. M. Heckl).
Chapter 15. Atomic Force Microscopy in the Study of Macromolecular Interact ions in Hemostasis and Thrombosis: Utility for Investigation of the Antiphospholipid Syndrome (William J. Montigny, Anthony S. Quinn, Xiao–Xuan Wu, Edwin G. Bovill, Jacob H. Rand, and Douglas J. Taatjes).
Index

Nota biograficzna:
BHANU P. JENA, PHD, DSc, is the George E. Palade University Professor and Distinguished Professor of Physiology at Wayne State University School of Medicine, Detroit, Michigan. In addition, Dr. Jena is Director of the University′s NanoBioScience Institute.
J. K. HEINRICH HÖRBER, PhD, is Professor in the Department of Physics at University of Bristol, Bristol, UK.

Okładka tylna:
A complete examination of the uses of the atomic force microscope in biology and medicine
This cutting–edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function.
The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X–ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore—the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers:Identification of the "porosome" in the growth hormone secreting cell of the pituitary glandProbing the structural and physical properties of microbial cell surfacesScanning probe microscopic characterization of the higher plant cell wall and its componentsCase studies of nano drug delivery systems using engineered dendrimersAFM techniques for studying living cellsInvestigating the intermolecular forces of leukocyte adhesion moleculesProtein–protein interactionsMicromechanical properties of lipid bilayers and vesicles
The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine.
This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.