Regulation of G Protein Coupled Receptor Function and Expression: Receptor Biochemistry and Methodology

G protein–coupled receptors (GPCRs) constitute the largest single class of receptors and are responsible for mediating much of intracellular mammalian signaling. Indeed, over 40 percent of the pharmacological targets being evaluated by drug companies are related to G protein–coupled receptors. Understanding how GPCRs are regulated is critical in determining waysto control their function and expression. This volume covers basic concepts while providing comprehensive treatment of methods used to examine how the functions of GPCRs are regulated.
Chapters in this volume, edited by Dr. Jeffrey Benovic, a world–renowned expert in the study of GPCR regulation, describe:
∗ Current techniques used to define the molecular mechanisms involved in receptor regulation
∗ ;Specific techniques employed to characterize receptors
∗ The role of regulatory proteins in modulating receptor function
∗ Methods used to measure receptor trafficking and expression
Covering a broad range of fundamental concepts and methodologies which are current in the study of GPCRs, Regulation of G Protein–Coupled Receptor Function and Expression is a vital reference for receptor biochemists, pharmacologists, neuroscientists, molecular biologists, cell biologists, and physiologists.
Also in this series:
Receptor Localization: Laboratory Methods and Procedures edited by Marjorie A. Ariano
Identification and Expression of G Protein–Coupled Receptors
edited by Kevin R. Lynch
Structure–Function Analysis of G Protein–Coupled Receptors
edited by Jürgen Wess

Spis treści:
TECHNIQUES FOR CHARACTERIZING RECEPTOR FUNCTION.
Epitope Tagging and Detection Methods for Receptor Identification (U. Klein & M. von Zastrow).
Measurement of Receptor Phosphorylation in Situ (M. Bouvier, et al.).
Reconstitution and in vitro Phosphorylation of G Protein–Coupled Receptors (J. Ptasienski & M. Hosey).
Identification of Residues that are Phosphorylated within a Receptor (K. Palczewski, et al.).
Palmitoylation of G Protein–Coupled Receptors (H. Jin, et al.).
Assays for Measuring Receptor/G Protein Coupling (Q. Li & R. Cerione).
PROTEINS THAT REGULATE RECEPTOR FUNCTION.
Expression and Activity of G Protein–Coupled Receptor Kinases (R. Loudon, et al.).
Characterization of Arrestin Expression and Function (V. Gurevich, et al.).
The G Protein–Coupled Receptor Phosphatase (J. Pitcher & R. Lefkowitz).
REGULATION OF RECEPTOR TRAFFICKING AND EXPRESSION.
Radioligand Binding Assays for G Protein–Coupled Receptor Internalization (M. Toews).
Characterization of Receptor Sequestration by Immunofluorescent Microscopy (F. Santini & J. Keen).
Characterization of Receptor Trafficking Using Green Fluorescent Protein (N. Tarasova, et al.).
The Analysis of Post–Transciptional Regulation of the Expression of G Protein–Coupled Receptors (B. Tholanikunnel & C. Malbon).
Index.

Okładka tylna:
G protein–coupled receptors (GPCRs) constitute the largest single class of receptors and are responsible for mediating much of intracellular mammalian signaling. Indeed, over 40 percent of the pharmacological targets being evaluated by drug companies are related to G protein–coupled receptors. Understanding how GPCRs are regulated is critical in determining waysto control their function and expression. This volume covers basic concepts while providing comprehensive treatment of methods used to examine how the functions of GPCRs are regulated.
Chapters in this volume, edited by Dr. Jeffrey Benovic, a world–renowned expert in the study of GPCR regulation, describe:
∗ Current techniques used to define the molecular mechanisms involved in receptor regulation
∗ ;Specific techniques employed to characterize receptors
∗ The role of regulatory proteins in modulating receptor function
∗ Methods used to measure receptor trafficking and expression
Covering a broad range of fundamental concepts and methodologies which are current in the study of GPCRs, Regulation of G Protein–Coupled Receptor Function and Expression is a vital reference for receptor biochemists, pharmacologists, neuroscientists, molecular biologists, cell biologists, and physiologists.
Also in this series:
Receptor Localization: Laboratory Methods and Procedures edited by Marjorie A. Ariano
Identification and Expression of G Protein–Coupled Receptors
edited by Kevin R. Lynch
Structure–Function Analysis of G Protein–Coupled Receptors
edited by Jürgen Wess