Practical HPLC Method Development

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high–performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Spis treści:
Getting Started.
Basics of Separation.
Detection Sensitivity and Selectivity.
Sample Preparation.
The Column.
Non–Ionic Samples: Reversed– and Normal–Phase HPLC.
Ionic Samples: Reversed–Phase, Ion–Pair, and Ion–Exchange HPLC.
Gradient Elution.
Systematic Approach to the Reversed–Phase Separation of Regular Samples.
Computer–Assisted Method Development.
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
Chiral Separations.
Preparative HPLC Separation.
Quantitation (Including Trace Analysis).
Completing the Method: Validation and Transfer.
Appendices.
Index.