Practical High–Performance Liquid Chromatography

Jump into the HPLC adventure!
Practical High–Performance Liquid Chromatography – FIFTH EDITION
Veronika R. Meyer
EMPA, St Gallen, Switzerland
Three decades on from publication of the 1st German edition of Veronika Meyer′s book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.
New sections on the following topics have been included in this fifth edition:Comparison of HPLC with capillary electrophoresisHow to obtain peak capacityvan Deemter curves and other coherencesHydrophilic interaction chromatographyMethod transferComprehensive two–dimensional HPLCFast separations at 1000 barHPLC with superheated water
In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.
A completely new chapter is presented on quality assurance covering:Is it worth the effort?Verification with a second methodMethod validationStandard operating proceduresMeasurement uncertaintyQualifications, instrument test, and system suitability testThe quest for quality
Reviews of earlier editions
"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst
“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." – American Journal of Therapeutics


Spis treści:
Contents
1. Introduction
1.1 HPLC: a powerful separation method
1.2 A first HPLC experiment
1.3 Liquid chromatographic separation modes
1.4 The HPLC instrument
1.5 Safety in the HPLC laboratory
1.6 Comparison between high–performance liquid chromatography and gas chromatography
1.7 Comparison between high–performance liquid chromatography and capillary electrophoresis
1.8 Units for pressure, length, and viscosity
1.9 Scientific journals
1.10 Recommended books
2. Theoretical Principles
2.1 The chromatographic process
2.2 Band broadening
2.3 The chromatogram and its purport
2.4 Graphical representation of peak pairs with different degree of resolution11
2.5 Factors affecting resolution
2.6 Extra–column volumes (dead volumes)15
2.7 Tailing
2.8 Peak capacity and statistical resolution probability19
2.9 Effects of temperature in hplc23
2.10 The limits of hplc
2.11 How to obtain peak capacity
3. Pumps
3.1 General requirements
3.2 The short–stroke piston pump
3.3 Maintenance and repair
3.4 Other pump designs
4. Preparation of equipment up to sample injection
4.1 Selection of mobile phase
4.2 Preparation of the mobile phase
4.3 Gradient systems
4.4 Capillary tubing
4.5 Fittings12
4.6 Sample injectors
4.7 Sample solution and sample volume
5. Solvent properties
5.1 table of organic solvents
5.2 Solvent selectivity
5.3 Miscibility
5.4 Buffers3
5.5 Shelf–life of mobile phases
5.6 The mixing cross
6. Detectors
6.1 General
6.2 UV detectors2
6.3 Refractive index detectors
6.4 Fluorescence detectors
6.5 Electrochemical (amperometric) detectors3
6.6 Light scattering detectors6
6.7 Other detectors
6.8 Multiple detection
6.9 Indirect detection
6.10 Coupling with spectroscopy
7. Columns and stationary phases
7.1 Columns for HPLC
7.2 Precolumns
7.3 General properties of stationary phases1
7.4 Silica4
7.5 Chemically modified silica6
7.6 Styrene–divinylbenzene13
7.7 Some other stationary phases
7.8 Column care and regeneration
8. HPLC column tests
8.1 Simple tests for HPLC columns
8.2 determination of particle size
8.3 Determination of breakthrough time
8.4 The test mixture
8.5 Dimensionless parameters for HPLC column characterization2
8.6 The van deemter equation from reduced parameters and its use in column diagnosis
8.7 diffusion coefficients3
9. Adsorption chromatography: normal–phase chromatography
9.1 What is adsorption?1
9.2 The eluotropic series
9.3 Selectivity properties of the mobile phase
9.4 Choice and optimization of the mobile phase
9.5 Applications
10. Reversed–phase chromatography
10.1 Principle
10.2 Mobile phases in reversed–phase chromatography
10.3 Solvent selectivity and strength8
10.4 Stationary phases
10.5 method development in reversed–phase chromatography15
10.6 Applications
10.7 Hydrophobic interaction chromatography15
11. Chromatography with chemically bonded phases
11.1 introduction
11.2 properties of some stationary phases
11.3 hydrophilic interaction chromatography6
12. Ion–exchange chromatography
12.1 Introduction
12.2 Principle
12.3 Properties of ion exchangers
12.4 Influence of the mobile phase
12.5 Special possibilities of ion exchange
12.6 Practical hints
12.7 Applications
13. Ion–pair chromatography
13.1 Introduction
13.2 Ion–pair chromatography in practice
13.3 Applications
13.4 Appendix: UV detection using ion–pair reagents5
14. Ion chromatography1
14.1 Principle
1 4.2 Suppression techniques
14.3 Phase systems3
14.4 Applications
15. Size–exclusion chromatography1
15.1 Principle
15.2 The calibration chromatogram
15.3 Molecular mass determination by means of size–exclusion chromatography5
15.4 Coupled size–exclusion columns
15.5 Phase systems
15.6 Applications
16. Affinity chromatography
16.1 Principle1
16.2 Affinity chromatography as a special case of hplc2
16.3 Applications
17. Choice of method
17.1 the various possibilities
17.2 method transfer8
18. Solving the elution problem
18.1 The elution problem
18.2 Solvent gradients2
18.3 Column switching7
18.4 Comprehensive two–dimensional hplc11
18.5 Optimization of an isocratic chromatogram using four solvents
18.6 Optimization of the other parameters
18.7 Mixed stationary phases
19. Analytical HPLC
19.1 Qualitative analysis1
19.2 Trace analysis
19.3 Quantitative analysis7
19.4 Recovery11
19.5 Peak–height and peak–area determination for quantitative analysis15
19.6 Integration errors
19.7 The detection wavelength
19.8 Derivatization20
19.9 Unexpected peaks: ghost and system peaks
20. Quality assurance1
20.1 Is it worth the effort?
20.2 Verification with a second method
20.3 Method validation3
20.4 Standard operating procedures (SOP’s)
20.5 Measurement uncertainty6
20.6 Qualifications, instrument test, and system suitability test
20.7 The quest for quality
21. Preparative hplc1
21.1 Problem
21.2 Preparative hplc in practice2
21.3 Overloading effects
21.4 Fraction collection
21.5 Recycling
21.6 Displacement chromatography10
22. Separation of enantiomers1
22.1 Introduction
22.2 Chiral mobile phases4
22.3 Chiral liquid stationary phases
22.4 Chiral solid stationary phases 6
22.5 Indirect separation of enantiomers16
23. Special possibilities
23.1 Micro, capillary and chip hplc1
23.2 High–speed and super–speed hplc7
23.3 Fast separations at 1000 bar: uplc9
23.4 HPLC with supercritical mobile phases11
23.5 HPLC with superheated water14
23.6 Electrochromatography15
24. Appendix 1: applied HPLC theory
25 Appendix 2: how to perform the instrument test
25.1 Introduction
25.2 Test sequence
25.3 Preparations
25.4 Pump test
25.5 UV detector test
25.6 Autosampler test
25.7 Column oven test
25.8 Equations and calculations
25.9 Documentation
26. Appendix 3: troubleshooting
26.1 Pressure problems
26.2 Leak in the pump system
26.3 Deviating retention times
26.4 Injection problems
26.5 Baseline problems
26.6 Peak shape problems
26.7 Problems with light scattering detectors (elsd)
26.8 Other causes
26.9 Instrument test
27. Appendix 4: column packing1


Okładka tylna:
Jump into the HPLC adventure!
Practical High–Performance Liquid Chromatography – FIFTH EDITION
Veronika R. Meyer
EMPA, St Gallen, Switzerland
Three decades on from publication of the 1st German edition of Veronika Meyer′s book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.
New sections on the following topics have been included in this fifth edition:Comparison of HPLC with capillary electrophoresisHow to obtain peak capacityvan Deemter curves and other coherencesHydrophilic interaction chromatographyMethod transferComprehensive two–dimensional HPLCFast separations at 1000 barHPLC with superheated water
In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.
A completely new chapter is presented on quality assurance covering:Is it worth the effort?Verification with a second methodMethod validationStandard operating proceduresMeasurement uncertaintyQualifications, instrument test, and system suitability testThe quest for quality
Reviews of earlier editions
"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst
“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." – American Journal of Therapeutics