Introducing Proteomics: From Concepts to Sample Separation, Mass Spectrometry and Data Analysis

Acknowledgements.

1 Introduction.

1.1 What are the Tasks in Proteomics?

1.2 Challenges in Proteomics.

1.3 Proteomics in Relation to Other –omics and System Biology.

1.4 Some General Applications of Proteomics.

1.5 Structure of the Book.

References.

2 Separation and Detection Technologies.

2.1 Introduction to Experimental Strategies in Proteomics.

2.2 Gel–Based Separation.

2.3 Visualization and Analysis of Proteins/Peptides in Gels.

2.4 Gel–Free Separation Technologies.

2.5. Visualization of Proteins/Peptides from Hyphenated Methods.

2.6 Chips in Proteomics Applications.

References.

3 Analysis of Peptides/Proteins by Mass Spectrometry.

3.1 Basic Principles of Mass Spectrometry for Proteomics.

3.2 Ionization Methods for Small Amounts of Biomolecules.

3.3 Mass Aanalyzers and Mass Spectrometers.

3.4 Concluding Remarks on Mass Analyzers for Proteomics.

References. 

4 Analysis and Interpretation of Mass Spectrometric and Proteomic Data.

4.1 Introduction.

4.2 Analysis of MS Data.

4.3 Analysis of MS/MS Data.

4.4 Quantification of LC MS and MS/MS Data from Complex Samples.

4.5 Bioinformatic Approaches for Mass Spectrometric Proteome Data Analysis.

References. 

5 Strategies in Proteomics.

5.1 Imaging Mass Spectrometry.

5.2 Qualitative Proteomics.

5.3 Differential and Quantitative Proteomics.

5.4 Analysis of Posttranslational Modifications.

5.5 Interaction–Proteomics.

5.6 Proteomics as Part of Integrated Approaches.

References.

Index.