Biointerface Characterization by Advanced IR Spectroscopy

IR spectroscopy has become without any doubt a key technique to answer questions raised when studying the interaction of proteins or peptides with solid surfaces for a fundamental point of view as well as for technological applications.

Principle, experimental set ups, parameters and interpretation rules of several advanced IR-based techniques; application to biointerface characterisation through the presentation of recent examples, will be given in this book. It will describe how to characterise amino acids, protein or bacterial strain interactions with metal and oxide surfaces, by using infrared spectroscopy, in vacuum, in the air or in an aqueous medium. Results will highlight the performances and perspectives of the technique.

Description of the principles, expermental setups and parameter interpretation, and the theory for several advanced IR-based techniques for interface characterisationContains examples which demonstrate the capacity, potential and limits of the IR techniquesHelps finding the most adequate mode of analysisContains examplesContains a glossary by techniques and by keywords

1. IRRAS and PM-IRRAS under ultra-vacuum conditions on metal surfaces . 2. PM-IRRAS at liquid interfaces. 3. Functionalization of semiconductor surfaces for biosensors or DNA attachment . 4. Biointerface Characterization by Advanced IR Spectroscopy. 5. Attenuated Reflection Infrared (ATR-IR) with Modulation Excitation Spectroscopy (MES), and Vibration Circular Dichroism (VCD). 6. Synchrotron Infrared Interface. 7. IR Spectroscopy for Biorecognition. 8. Advanced infrared glasses for biochemical sensing. 9. AFM-IR: photothermal infrared nanospectroscopy; Application to cellular imaging. 10. Sum-Frequency Generation Spectrocopy of Biointerfaces. Synthesis and comparison of various IR-based techniques