Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.

This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnologyFeatures clear, step-by-step instruction for applying the techniques coveredOffers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

1. Introduction to Laboratory Practices
2. Dos and Don'ts in the Lab
3. Equipment Care
4. Agarose Gel Electrophoresis
5. SDS-PAGE
6. DNA plasmid isolation using Alkaline Lysis Method from E.coli DH5α cells
7. Restriction digestion of Plasmid DNA
8. Genomic DNA extraction from plant leaves using CTAB method
9. Genomic DNA extraction from Silk Worm using modified phenol: chloroform method (Suzuki et al., 1972)
10. Genomic DNA extraction from Fungal Mycelium using modified Cenis, 1992 method
11. Bacterial transformation protocol using CaCl2
12. Centrifugation- Principle and Types
13. Various Chromatographic techniques
14. Thin Layer Chromatography
15. Gel Filtration/Size Exclusion Chromatography
16. Ion Exchange Chromatography
17. Affinity Chromatography
18. Paper Chromatography
19. Growth of bacterial cultures and preparation of growth curve
20. Polymerase Chain Reaction 
21. Blotting/ Hybridization
22. Dot Blot Analysis
23. ELISA
24. Standard Operating Procedures (SOPs) for various equipment