Odorant Binding and Chemosensory Proteins

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

1. Genome mining and sequence analysis of chemosensory soluble proteins in arthropods

Joel Vizueta, Paula Escuer, Alejandro Sánchez-Gracia and Julio Rozas

2. Reconstructing protein coding sequences from ancient DNA

Michael Hofreiter and Stefanie Hartmann

3. Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs

Jean-François Picimbon

4. Proteomics of arthropod soluble olfactory proteins

Francesca R. Dani and Giuseppe Pieraccini

5. Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects

Andrea Scaloni

6. Bacterial expression and purification of vertebrate odorant-binding proteins

Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loïc Briand

7. Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography

Anaïs Gaubert, Béatrice Amigues, Silvia Spinelli and Christian Cambillau

8. Solution structure of insect CSP and OBPs by NMR

Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone

9. Stability of OBPs

Nadja Hellmann

10. Ligand-binding assays with OBPs and CSPs

Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi

11. Fine structure immunocytochemistry—An important tool for research on odorant-binding proteins

Rudolf Alexander Steinbrecht

12. Effect of OBPs on the response of olfactory receptors

Bing Wang, Song Cao, Wei Liu and Guirong Wang

13. Site-directed mutagenesis of odorant-binding proteins

Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi

14. Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing

Yusuke Shiota and Takeshi Sakurai

15. Electrochemical biosensing with odorant binding proteins

Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu

16. Electronic biosensors based on graphene FETs

Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll

17. Electronic biosensors based on EGOFETs

Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli

18. Gravimetric biosensors

Khasim Cali, Elena Tuccori and Krishna C. Persaud

19. Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins