Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing ß-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.

1. Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation

Ting Jiang and Scott T. Laughlin

2. Analysis of metabolically labeled inositol phosphate messengers by NMR

Robert Puschmann, Robert K. Harmel and Dorothea Fiedler

3. Photo-releasable derivatives of inositol pyrophosphates

Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz

4. IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation

Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin

5. Fluorescent bioorthogonal labeling of class B GPCRs in live cells

Srikanth Kumar Gangam and Qing Lin

6. Near-infrared photoactivatable nitric oxide donors with photoacoustic readout

Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan

7. H₂S donors with optical responses

Michael D. Pluth, Yu Zhao and Matthew M. Cerda

8. Visualization of oxidative stress-induced carbonylation in live mammalian cells

Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane

9. Receptor-based fluorescent sensors constructed from ribonucleopeptide

Skun Nakano, Hiroaki Konishi and Takashi Morii

10. Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy

Siddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann

11. A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore

Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann

12. Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay

Kirsten Deprey and Joshua A. Kritzer

13. Cell-penetrating and mitochondrion-targeting molecules

George Appiah Kubi and Dehua Pei

14. A photocaged DNA nanocapsule for delivery and manipulation in cells

Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo

15. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells

Esther Braselmann and Amy E. Palmer

16. Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)

Kayla N. Busby and Neal K. Devaraj

17. Assaying RNA solvent accessibility in living cells with LASER

Chao Feng and Robert C. Spitale

18. Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics

Jeffrey A. Dewey and Bryan C. Dickinson

19. Cross-linking cellular nucleic acids via a target-directing double click reagent

Masayuki Tera and Nathan W. Luedtke

20. Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes

Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard

21. Quantification of protein delivery in live cells using fluorescence correlation spectroscopy