Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

1. Fluorescent detection of peroxynitrite during antibody-dependent cellular phagocytosis

Digamber Rane, Erick Carlson, Yuwen Yin and Blake R. Peterson

2. Synthesis, evaluation, and biological applications of visible-light-controllable nitric oxide releasers

Naoya Ieda and Hidehiko Nakagawa

3. Design of fluorescent protein-based sensors through a general protection-deprotection strategy

Gloricelly M. Roman-Arocho, Xin Shang, Wei Niu and Jiantao Guo

4. Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP

Andrew B. Dippel, Wyatt A. Anderson, Michael M. Maiden, Chris Waters and Ming C. Hammond

5. Calcium-responsive liposomes: Toward ion-mediated targeted drug delivery

Jinchao Lou and Michael D. Best

6. Far-red photoactivatable BODIPYs for the super-resolution imaging of live cells

Yang Zhang, Sicheng Tang, Laura Ravelo, Janet Cusido and Françisco M. Raymo

7. Washington Red (WR) dyes and their imaging applications

Wei Chen and Ming Xian

8. Bioluminescence imaging in mice with synthetic luciferin analogues

Xincai Ji, Spencer T. Adams and Stephen C. Miller

9. Monitoring neuronal activity using PeT voltage sensitive dyes

Molly J. Kirk, Benjamin K. Raliski and Evan Miller

10. Temporally gated molecular tools for tracking protein-protein interactions in live cells

Kayla E. Kroning and Wenjing Wang

11. trLRET microscopy: Ultrasensitive imaging of lanthanide luminophores

Paulina Ciepla, Ukrae Cho and James K. Chen

12. Long fluorescence lifetime triangulenium dyes in imaging and fluorescence polarization assay

Bo W. Laursen, Sidsel A. Bogh and Thomas Just Sørensen

13. Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl-selenium chemistry

Woohyun Lee, Sandip V. Mulay, Shingo Shimodaira, Ariq Abdillah, Jaymee Palma, Youngsam Kim, Tesla Yudhistira and David G. Churchill

14. Detection of cell-surface phosphatidylserine using the fluorogenic probe P-IID

Vincent E. Zwicker, Jia Hao Yeo, Stuart T. Fraser, Elizabeth J. New and Katrina A. Jolliffe

15. Quinoline-based fluorescent small molecules for live cell imaging