Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.

1. Fluorogenic detection of protein aggregates in live cells using the AggTag method

Kwan Ho Jung and Xin Zhang

2. Synthesis and application of a ratiometric probe for hydrogen peroxide

Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains

3. Protein labeling for FRET with methoxycoumarin and acridonylalanine

Chloe M. Jones, Yarra Venkatesh and E. James Petersson

4. Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays

Paul T. Ludford III and Yitzhak Tor

5. Method to discriminate amyloids using fluorescent probes

Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang

6. Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives

Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas

7. Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer

John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo

8. Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles

Nanxi Wang and Lei Wang

9. 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy

Arusha Acharyya, Ismail A. Ahmed and Feng Gai

10. Dissecting contributions of catalytic and reader domains in regulation of histone demethylation

Nektaria Petronikolou, James E. Longbotham and Danica Galonić Fujimori

11. Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization

Qing Zhang, Yue Zhang and Xuechen Li

12. Chemical synthesis of di-ubiquitin modified histones for further biochemical studies

Jia-Bin Li, Jun Liang and Changlin Tian

13. Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies

Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis

14. FlICk (fluorescent isoindole crosslinking) for peptide stapling

Mihajlo Todorovic and David M. Perrin

15. Selective protein N-terminal labeling with N-hydroxysuccinimide esters

Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey

16. Covalent live-cell labeling of proteins using a photoreactive fluorogen

Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra

17. Photoconvertible diazaxanthilidene dyes for live cell imaging

Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth

18. No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag