Methods in Kidney Cell Biology Part B

Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.

Written by experts in their field who have perfected stated methodsCovers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined formIncludes cutting-edge, recently developed methods

1. Single glomerular proteomics – a novel tool for translational glomerular cell biology Markus Rinschen  2. Kidney harvesting and metabolite extraction for metabolomics studies in rodents Maria Irazabal Mira  3. Optical tissue clearing and immunolabeling in kidney research David Ellison 4. Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease Roman Müller  5. Intravital Visualization of the Primary Cilium, Tubule Flow, and Innate Immune Cells in the Kidney Utilizing an Abdominal Window Imaging Approach Bradley Yoder 6. Novel fluorescence techniques to quantitate renal cell biology Janos Peti-Peterdi  7. Manipulation of Renal Gene Expression Using Oligonucleotides Vishal Patel 8. Methods for renal lineage tracing: in vivo and beyond Thomas Carroll 9. In Vivo Magnetic Resonance Imaging Techniques for Structural and Functional Characterization of Murine Model Kidneys Tim Kline 10. In vivo analysis of renal epithelial cells in zebrafish Zhaoxia Sun 11. Visualizing gene expression during zebrafish pronephros development and regeneration Rebecca Wingert 12. Drosophila melanogaster and its nephrocytes – a versatile model for glomerular research Paul Brinkkotter