Retinoid Signaling Pathways

Retinoid Signaling Pathways, Volume 637, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sections in this release include The chemistry and biochemistry of Vitamin A and its natural derivative, Biosynthesis of retinoic acids, Biodegration of retinoic acids mediated by retinoid binding proteins, Retinoic acid homeostasis, Cryo Electron Microscopy to study retinol update via the STRA6 receptor, Immuno-detection of retinoic acid synthesis enzymes in the brain, classical pathway of gene regulation by retinoids, Protein-protein interactions in the regulation of retinoid acid receptors activity, and much more.

1. Vitamin A and its natural derivatives

Chris P.F. Redfern

2. Post-natal all-trans-retinoic acid biosynthesis

Joseph L. Napoli

3. Retinoid metabolism and functions mediated by retinoid binding-proteins

Joseph L. Napoli and Hong Sik Yoo

4. Generation and isolation of recombinant retinoid oxidoreductase complex

Mark K. Adams, Olga V. Belyaeva and Natalia Y. Kedishvili

5. Sample preparation for structural and functional analyses of the STRA6 receptor for retinol-binding protein

Brianna K. Costabile, Youn-Kyung Kim, Yunting Chen, Oliver B. Clarke, Loredana Quadro and Filippo Mancia

6. Use of fixatives for immunohistochemistry and their application for detection of retinoic acid synthesizing enzymes in the central nervous system

Azita Kouchmeshky and Peter McCaffery

7. Classical pathways of gene regulation by retinoids

Ehmke Pohl and Charles W.E. Tomlinson

8. Protein-protein interactions in the regulation of RAR–RXR heterodimers transcriptional activity

Albane le Maire, Pierre Germain and William Bourguet

9. Natural ligands of RXR receptors

Patricia García, Paula Lorenzo and Angel R. de Lera

10. DNA recognition by retinoic acid nuclear receptors

Carole Peluso-Iltis, Judit Osz and Natacha Rochel

11. Noncanonical retinoic acid signaling

Jennifer Nhieu, Yu-Lung Lin and Li-Na Wei

12. Retinoic acid and microRNA

Lijun Wang, Atharva Piyush Rohatgi and Yu-Jui Yvonne Wan

13. Analysis of vitamin A and retinoids in biological matrices

Lindsay C. Czuba, Guo Zhong, King Yabut and Nina Isoherranen

14. Effects of vitamin A and retinoic acid on mouse embryonic stem cells and their differentiating progeny

Mary Ann Asson-Batres and Christopher W. Norwood

15. Identifying vitamin A signaling by visualizing gene and protein activity, and by biochemical isolation of vitamin A metabolites

Stephen R. Shannon, Jianshi Yu, Amy E. Defnet, Danika Bongfeldt, Alexander R. Moise, Maureen A. Kane and Paul A. Trainor

16. Whole mount in situ hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus

Matteo Bozzo, Simona Candiani and Michael Schubert

17. Design of synthetic retinoids

David R. Chisholm and Andrew Whiting

18. Application of human organotypic skin raft cultures for analysis of retinoid metabolism, retinoic acid signaling, and screening of bioactive rexinoids

Alla V. Klyuyeva, Kelli R. Goggans, Natalia Y. Kedishvili and Olga V. Belyaeva

19. Tissue localization of retinoic acid receptor (RAR) active drugs

Azita Kouchmeshky, Timothy Goodman, Andy Whiting and Peter McCaffery

20. The development of methodologies for high-throughput retinoic acid binding assays in drug discovery and beyond

Charles W.E. Tomlinson and Andrew Whiting

21. Using the human CYP26A1 gene promoter as a suitable tool for the determination of RAR-mediated retinoid activity