Tumor Immunology and Immunotherapy - Integrated Methods Part A

Tumor Immunology and Immunotherapy Integrated Methods - Part A, Volume 635 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry, High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers, Multiplex assay by IHC for melanoma tumor microenvironment evaluation, Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images, Phenotyping of immune cells in situ using multispectral imaging quantification, and much more.

1. High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers Grace Banik, Courtney B. Betts, Shannon M. Liudahl, Shamilene Sivagnanam, Rie Kawashima, Tiziana Cotechini, William Larson, Jeremy Goecks, Sara I. Pai, Daniel R. Clayburgh, Takahiro Tsujikawa and Lisa M. Coussens 2. Multiplex immunohistochemistry assay to evaluate the melanoma tumor microenvironment Giosuè Scognamiglio, Mariaelena Capone, Domenico Mallardo, Gerardo Botti, Paolo A. Ascierto and Gabriele Madonna 3. Characterization of the immune microenvironment of NSCLC by multispectral analysis of multiplex immunofluorescence images Michael Surace, Lorenz Rognoni, Jaime Rodriguez-Canales and Keith E. Steele 4. Multiplexed immunohistochemistry for immune cell phenotyping, quantification and spatial distribution in situ Angela Vasaturo and Jérôme Galon 5. Multiplex immunohistochemistry as a novel tool for the topographic assessment of the bone marrow stem cell niche Marcus Bauer, Christoforos Vaxevanis, Daniel Bethmann, Chiara Massa, Nikolaos Pazaitis, Claudia Wickenhauser and Barbara Seliger 6. Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry Ileana S. Mauldin, Natasha D. Sheybani, Samuel J. Young, Richard J. Price and Craig L. Slingluff 7. Tracing bone marrow-derived microglia in brain metastatic tumors Wei Du and Paula D. Bos 8. Monitoring abscopal responses to radiation in mice Maria Esperanza Rodriguez-Ruiz, Takahiro Yamazaki, Aitziber Buqué, Norma Bloy, Viviane A. O. Silva, Lena Stafford, Ai Sato and Lorenzo Galluzzi 9. Methods to assess anticancer immune responses in orthotopic bladder carcinomas Randy F. Sweis 10. Characterization of conventional dendritic cell populations in preclinical tumor models using flow cytometry Erik Wennerberg, Nils Rudqvist and Sheila Spada 11. Quantitative evaluation of tumor-specific T cells in tumors and lymphoid tissues Kathleen M. Kokolus, Nataša Obermajer and Pawel Kalinski 12. Generation and validation of recombinant herpes simplex type 1 viruses (HSV-1) using CRISPR/Cas9 genetic disruption Praveen K. Bommareddy, Cole Peters and Howard L. Kaufman 13. Production and use of adeno-associated virus vectors as tools for cancer immunotherapy Myriam Fernandez-Sendin, Shirley Tenesaca, Marcos Vasquez, Fernando Aranda and Pedro Berraondo 14. Modulation of the tumor microenvironment with an oncolytic adenovirus for effective T-cell therapy and checkpoint inhibition João Manuel Santos, Riikka Havunen and Akseli Hemminki 15. Genetic modification of oncolytic viruses to enhance antitumor immunity Maria Eugenia Davola, Alyssa Vito, Jiarun Wei, Nader El-Sayes, Samuel Workenhe and Karen Louise Mossman 16. CRISPR technology for immuno-oncology applications Jianbin Wang, Vitaly Balan and Francesco Marincola 17. Neoantigen prediction from genomic and transcriptomic data Sebastiano Battaglia 18. Monitoring antigen cross-presentation with human dendritic cells purified from the blood Stephane Isnard, Etienne Hatton, Jean-Baptiste Guillerme and Anne Hosmalin