Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part B

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics

1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori 2. Design and synthesis of fluorescent activity probes for protein phosphatases Garrett R. Casey, Jon R. Beck and Cliff I. Stains 3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells Travis J. Nelson, Jia Zhao and Cliff I. Stains 4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation Justin Lawrie, Wei Niu and Jiantao Guo 5. Synthesis and screening of bead-displayed combinatorial libraries Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek 6. Visualizing the brain's astrocytes Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin 7. Modular activatable bioorthogonal reagents Pratik Kumar and Scott T. Laughlin 8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors Sam Lear, Zaid Amso and Weijun Shen 9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi 10. Selection and optimization of enzyme reporters for chemical cytometry Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton 11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactions Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow 12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu 13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting Narek Darabedian and Matthew R. Pratt 14. Optogenetic perturbation of the biochemical pathways that control cell behavior Lauren L. Haar, David S. Lawrence and Robert M. Hughes 15. pH-shift stress on antibodies Hiroshi Imamura and Shinya Honda 16. Small molecule target identification using photo-affinity chromatography Seung-Yong Seo and Timothy W. Corson 17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai 18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi 19. Bioorthogonal oncometabolite ligation Chloe A. Briney, Susana Najera and Jordan L. Meier