Biological NMR Part B

Biological NMR, Part B, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Protein methyl labeling, Membrane protein expression – yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs & micelles MP host, PTM – phosphorylation, PTM – lipidation, Screening platform for receptor-ligand discovery, Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration, Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, and much more.

1. Companion Simulations and Modeling to NMR-Based Dynamical Studies of Proteins Kim A. Sharp 2. Reverse Micelle Encapsulation of Proteins for NMR Spectroscopy Brian Fuglestad, Bryan S. Marques, Christine Jorge, Nicole E. Kerstetter, Kathleen G. Valentine and A. Joshua Wand 3. Characterizing Protein Hydration Dynamics Using Solution NMR Spectroscopy Christine Jorge, Bryan S. Marques, Kathleen G. Valentine and A. Joshua Wand 4. Understanding Protein Function Through an Ensemble Description Characterization of Functional States by ¹⁹F NMR Christopher Di Pietrantonio, Aditya Pandey, Jerome Gould, Advait Hasabnis and Robert Scott Prosser 5. Overhauser Dynamic Nuclear Polarization for the Study of Hydration Dynamics, Explained John M. Franck and Songi Han 6. Chemical Exchange Arthur G. Palmer III and Hans Koss 7. Characterization of Internal Protein Dynamics and Conformational Entropy by NMR Relaxation Matthew A. Stetz, José A. Caro, Sravya Kotaru, Xuejun Yao, Bryan Marques, Kathleen G. Valentine and A. Joshua Wand 8. NMR Methods for Characterizing the Basic Side Chains of Proteins: Electrostatic Interactions, Hydrogen Bonds, and Conformational Dynamics Dan Nguyen, Chuanying Chen, B. Montgomery Pettitt and Junji Iwahara 9. Solid-State NMR Spectroscopy of RNA Alexander Marchanka and Teresa Carlomagno 10. DNP-Assisted NMR Investigation of Proteins at Endogenous Levels in Cellular Milieu Whitney N. Costello, Yiling Xiao and Kendra K. Frederick 11. Identification of Unknown Metabolomics Mixture Compounds by Combining NMR, MS, and Cheminformatics Abigail Leggett, Cheng Wang, Da-Wei Li, Arpad Somogyi, Lei Bruschweiler-Li and Rafael Brüschweiler 12. STD NMR as a Technique for Ligand Screening and Structural Studies Samuel Walpole, Serena Monaco, Ridvan Nepravishta and Jesus Angulo 13. An ELISA-Based Screening Platform for Ligand-Receptor Discovery Sinem Ozgul, Sventja von Daake, Sumie Kakehi, Davide Sereni, Natalia Denissova, Carlie Hanlon, Yuanpeng Janet Huang, John K. Everett, Cuifeng Yin, Gaetano T. Montelione and Davide Comoletti 14. Protein–Small Molecule Interactions by waterLOGSY Renjie Huang and Ivanhoe K.H. Leung 15. Applications of Dissolution-DNP for NMR Screening Yaewon Kim and Christian Hilty