Light and Video Microscopy

Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope.

Provides a fully-revised, updated resource on three-dimensional (3D) structures Contains a new appendices on Diffraction Theory and Advanced Image ProcessingProvides practical applications, lab exercises and case studies on the mathematics, physics and biology used in microscopyDiscusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscopes, oblique illumination and photomicrography

1. The Relation Between the Object and the Image
2. Geometrical Optics
3. Physical Optics
4. Bright Field Microscopy
5. Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination
6. Photomicrography
7. Fluorescence Microscopy
8. Polarization Microscopy
9. Interference Microscopy
10. Differential Interference Microscopy
11. Modulation Contrast Microscopy
12. Video and Digital Microscopy
13. Image Processing and Analysis
14. Various Kinds of Microscopes and Accessories