AGO-Driven Non-Coding RNAs: Codes to Decode the Therapeutics of Diseases

The ‘RNA world’ hypothesis that proposed RNA molecules as the first form of genetic material was put forwarded in the late 1980s but got impetus only recently when high-throughput sequencing technologies began unearthing new types of non-coding RNA (ncRNA) transcripts in higher eukaryotes. Till then, research on ncRNAs were primarily confined to transfer RNAs and, ribosomal RNAs, which act as the messengers of the protein synthesis and allow translation of genetic information encoded by DNA into proteins. In recent years, the integration of high-throughput genomic technologies with molecular biology and omics sciences have revolutionized the fields of ncRNA research by identifying the hidden treasures of several new types of ncRNAs encoded in the genomes of several organisms and decrypting their versatile roles in gene expression and epigenetics. Among these, two small endogenous ncRNAs, namely microRNAs (miRNAs) and piwi-interacting RNAs (piRNAs) that drive argonaute (AGO) family of proteins namely Ago and Piwi respectively and silence the expression of genes have geared up molecular and disease biology research in recent years. Both miRNAs and piRNAs are expressed in higher eukaryotes, including human and act as cellular rheostats by regulating the expression of significant fraction of genes encoded in the genomes. The aberrant expressions of these small ncRNAs within the cells cause various abnormalities and diseases including cancer. Manipulating their aberrant expression or function can serve as potential novel diagnostic/prognostic biomarkers and bring in new therapeutic strategies for multiple human diseases. This can be further translated from bench-side to clinic for improving human health.

This book captures the essence of the pioneering work of some of the world’s leading researchers showcasing the scientific excitements surrounding the evolving regulatory roles of miRNAs and piRNAs highlighting their potential towards the diagnosis and therapeutics of various diseases. The book is geared towards scientists, students, and will particularly appeal to active investigators in RNA biology, molecular biology, cancer research as well as clinicians and will provide them a comprehensive view of recent discoveries and research progresses to utilize miRNAs, piRNAs and their interacting proteins, Ago and Piwi for diagnosis, prognosis and therapeutics of diseases.

Provides a unified cutting-edge resource for both miRNAs and piRNAs, two promising AGO-clade small ncRNAs, their functions and potential applicationsShowcase high-throughput technologies and other approaches for discovery of these small ncRNAs and their targetsUnveils the diverse molecular mechanisms by which miRNAs and piRNAs regulate gene expression in animal cellsShowcase recent discoveries on involvement of Argonaute and Piwi proteins in different biological processes and diseases as well as their possible use in diagnosisReport breakthroughs in the use of small ncRNAs for diagnosis and personalized therapy

1. Two small non-Coding RNAs, Two different mechanisms: MicroRNAs and PIWI Interacting RNAs-From Origin to Cancer 2. Computational Methods for miRNA & piRNA Gene Discovery and Functional Predictions 3. The role of microRNAs in bacterial infections 4. MiRNAs shaping cellular reprogramming 5. Small RNA proteome as disease biomarker: An incognito treasure of clinical utility 6. MicroRNAs in diagnosis and therapeutics 7. Small non-coding RNAs as a tool for personalized therapy in familial cancers 8. Roles of microRNAs in neurodegeneration: lessons from Parkinson's disease models 9. Role of miRNAs in cardiovascular diseases and their therapeutic implications 10. microRNA therapeutics for Glioblastoma: Candidates and Targeting Strategies 11. Emerging roles of miRNAs as a regulator in progression of lung cancer and their implications in its diagnosis and therapy 12. Rejuvenation of piRNAs in emergence of cancer hallmarks 13. piRNAs in the pathophysiology of disease and potential clinical applications