Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration.

Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more.

Section 1 - Microfluidics for micro-organisms 1. Temperature control and drug delivery for cell division cycle control in fission yeast 2. H2O2 stress response in budding yeast 3. Antibiotic resistance in bacteria 4. Metabolism in bacteria 5. Fluidized beds for bacterial sorting and amplification

Section 2 - Microfluidics for cell culture and cell sorting of mammalian cells 6. Hydrogel microwells 7. Cell sorting with conical filters 8. Directed neuronal cell culture

Section 3 - Microfluidics for cell migration 9. Immune cells migration in complexe environments 10. Neutrophiles migration in health and disease 11. Cell guidance by physical cues 12. Stable gradients in gels of extracellular matrix for cancer cell migration

Section 4 - Microfluidics for cell mechanics 13. Size based cell sorting and single cell mechanobiology 14. High throughput measure of single cell deformability/stiffness 15. Host-microbe interactions 16. Cell confinement and intracellular crowding

Section 5 - Microfluidics for Single cell analytics 17. Quantification of single cell secretion 18. Mechanical cell disruption