Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry

Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data.

Instructors will find this book useful because of the modular nature of the lab exercises included. Written in a logical, easy-to-understand manner, this book is an indispensable resource for both students and instructors.

Offers project lab formats for students that closely simulate original research projectsProvides instructional guidance for students to design their own experimentsPresents advanced analytical techniquesIncludes access to a website with additional resources for instructors

1. The Structure and Function of Secondary Metabolites that are Secreted by Bacteria 2. The Reagents, Supplies and Equipment that are Necessary to Grow Cultures of Bacteria in the Laboratory and to Purify Secreted Metabolites 3. Overview of the Methods for Purification of Metabolites that are Secreted by Bacteria 4. Absorption Spectrophotometry: Ultraviolet-visible and Infrared 5. High-performance Liquid Chromatography 6. Mass Spectrometry 7. X-ray Crystallography 8. Nuclear Magnetic Resonance Spectroscopy 9. Exercises in Purifying and Characterizing a Quorum-sensing Signal 10. Exercises in Purifying and Characterizing Iron-chelating Molecules 11. Exercises in Purifying and Characterizing a Chloroplast-targeting Phytotoxin 12. Designing your Own Experiments