Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems, Second Edition applies the design and experimental techniques used in MEMS microfabrication and microfluidics to cell culture systems. The book serves as a professional reference, providing a practical guide to the design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. It covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. This updated edition contains new material that strengthens the focus on in vitro models that are useful for drug discovery and development.

New sections in this edition review liver organ models from an industry perspective, while other material highlights the development of organ models and systems for specific applications in disease modeling and drug safety.

Provides design and operation methodologies for microfluidic and microfabricated biomaterials approaches to tissue engineering and cell culture systemsComprehensively covers strategies and techniques, ranging from academic first principles, to industrial scale-up approachesOffers design principles, operation techniques and background information/perspectives

Part 1: Introduction 1. Microfluidic Cell Culture Technologies 2. Inertial microfluidics for stem cell isolation 3. Microfluidic Structures for Controlling Stem Cell Microenvironments 4. Microfluidic cell culture platforms with embedded nanoscale features 5. Microfluidic Vascular Networks for Engineered Tissues 6. Direct Write Assembly of Organ Models 7. 3D Bioprinting for Organ Models

Part II: Organ-Specific Disease and Drug Safety Models 8. Microfabricated Kidney Tissue Models 9. Microfluidic Hepatotoxicity Platform 10. Dynamic Gastrointestinal and Pulmonary Organ Models 11. Cardiac Models 12. Neural Tissue Microphysiological Systems 13. Models of the Female Reproductive Tract 14. Microscale Models of Inflammatory Diseases 15. Blood-Retinal Barrier-on-a –chip 16. Human blinking eye-on-a chip 17. Circulating tumor cell cultures for evaluation of anticancer treatment

Part III: Technologies for Sensing, Multiplexed and Interconnected Organ Models 18. Live Cell Analysis Under Shear Flow 19. High Throughput Flow Systems for Cardiovascular Research 20. Phaseguide technology for connected organ models 21. Electromagnetically microactuated platforms for dynamic microphysiological systems 22. Interconnected organ models for skin and liver 23. Heart-Liver-Vascular Interconnected Microphysiological Systems 24. Rocking Platform Technology for Interconnected Organ Models