Organoids and Mini-Organs

Organs and Organoids combines contributions from leading practitioners who work under the editorial control of an acclaimed researcher who also served for eight years as Editor-in-Chief of the journal Organogenesis, the first journal on this topic. The book begins with an introduction, but then delves into chapters that present advice on how to make organoids for many systems. In addition, case studies that illustrate the uses of organioids are presented, along with discussions on future directions and specific problems that need to be solved.

Collects the best protocols of organoid cultures from diverse tissuesCovers a wide range of organsIncludes troubleshooting cases for common, but specific problems for each culture conditionsProvides an entire section on the application of organoids

PART I INTRODUCTION 1. Organoids and mini-organs: Introduction, history, and potential

PART II CONSTRUCTING ORGANOIDS 2. Elements of organoid design 3. Intestinal organoids: Mini-guts grown in the laboratory 4. Three-dimensional breast culture models 5. Prostate organoids: Directed differentiation from embryonic stem cells 6. Kidney organoids 7. Spontaneous self-assembly of liver organoids from differentiated human cells 8. Cerebral organoids: Building brains from stem cells 9. From organoids to mini-organs: A case study in the kidney

PART III APPLICATIONS OF ORGANOIDS 10. Intestinal organoids: A model for biomedical and nutritional research 11. A three-dimensional neurosphere system using human stem cells for nanotoxicology studies 12. Organoids for modeling kidney disease 13. Intestinal organoids in studying host2bacterial interactions 14. Four challenges for organoid engineers