Correlative Light and Electron Microscopy III

Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics.

Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.

Contains contributions from experts in the fieldCovered topics include targeted ultramicrotomy and high-precision correlationPresents recent advances and currently applied correlative approachesGives detailed protocols allowing the application of workflows in one’s own laboratory settingCovers CLEM approaches in the context of specific applicationsAims to stimulate the use of new combinations of imaging modalities

1. Millisecond time-resolved CLEM 2. Super resolution LM und SEM of high-pressure frozen C. elegans 3. Preservation fluorescence, super res CLEM 4. Targeted ultramicrotomy 5. CEMOVIS-CLEM 6. APEX in Tissue 7. Corrsight mit IBIDI flowthrough chamber 8. Corrsight 9. Correlative Light Atomic Force Electronic Microscopy (CLAFEM) 10. Atmospheric EM CLEM 11. Correlated Fluo Light SEM 12. Near infrared branding + SBF SEM 13. 4. Correlative MicroCT 14. High-precision correlation 15. CLEM acquisition 16. ecCLEM mapping software