Cytokinesis

Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.

1. Labeling Membrane Domains in Dividing Echinoderm Eggs David Burgess 2. Dynamics of sphingomyelin- and cholesterol-enriched lipid domains during cytokinesis Toshihide Kobayashi 3. Analysis of Protein Dynamics During Cytokinesis in Budding Yeast Erfei Bi 4. Imaging cytokinesis of Drosophila S2 cells Gilles Hickson 5. Studying cytokinesis in Drosophila epithelial tissues Yohanns Bellaïche 6. Visualization of cleavage furrow proteins in fixed dividing spermatocytes Maria Grazia Giansanti 7. Monitoring complete and incomplete abscission in the germline stem cell lineage of Drosophila ovaries J R. Huyhn 8. Incomplete Cytokinesis / Binucleation in Mammals: the powerful system of Hepatocytes. Chantal Desdouets 9. Post-abscission membrane dynamics associated with cytokinesis – basal process splitting and midbody release Wieland B. Huttner 10. Understanding the role of cytokinesis and midbody formation during epithelial cell polarization Rytis Prekeris 11. Micromanipulation of daughter cells for the study of cytokinetic abscission Matthieu Piel 12. Measuring Abscission Spatiotemporal Dynamics using Quantitative High Resolution Microscopy Natalie Elia 13. Analysis of cytokinesis by electron microscopy Thomas Müller-Reichert 14. Studying cytokinesis and midbody remnants using correlative light/scanning EM Arnaud Echard 15. Analysis of Post-cytokinetic Roles of Cytokinetic Components in C. elegans Guangshuo Ou 16. Variations on a theme: Imaging cytokinetic and stable rings in situ using C. elegans Amy Shaub Maddox 17. Using fast-acting temperature sensitive mutants to study cell division in C. elegans Julie C. Canman 18. Cytokinesis from nanometers to micrometers and microseconds to minutes Douglas N. Robinson 19. An active contour ImageJ plugin to monitor daughter cell size in 3D during cytokinesis Ewa Kamila Paluch 20. Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast Sophie Martin 21. Single molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers Martin Loose 22. Preparation of centralspindlin as an active heterotetramer of kinesin and GAP subunits for in vitro structural and functional assays Masanori Mishima 23. In vitro reactivation of the cytokinetic contractile ring of fission yeast cells Issei Mabuchi 24. Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis Christine Field