Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion.

Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymologyContains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

1. Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases T.L. Pavankumar, J.C. Exell and S.C. Kowalczykowski 2. Single Molecule Imaging with one Color Fluorescence Y. Qiu and S. Myong 3. Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy R. Chadda and J.L. Robertson 4. Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging S.R. Hansen, M.L. Rodgers and A.A. Hoskins 5. Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy E.M. Boehm, S. Subramanyam, M. Ghoneim, M.T. Washington and M. Spies 6. How to Measure Separations and Angles Between Intra-Molecular Fluorescent Markers K.I. Mortensen, J. Sung, J.A. Spudich and H. Flyvbjerg 7. Precisely and Accurately Inferring Single-Molecule Rate Constants C.D. Kinz-Thompson, N.A. Bailey, R.L. Gonzalez 8. Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout M.E. Moses, P. Hedegård and N.S. Hatzakis 9. Putting Humpty-Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome C.E. Rohlman, M.R. Blanco and N.G. Walter 10. Single Molecule FRET to Measure Conformational Dynamics of DNA Mimatch Repair Proteins J.W. Gauer, S. LeBlanc, P. Hao, R. Qiu, B. Case, M. Sakato, M.M. Hingorani, D.A. Erie and K.R. Weninger 11. Single Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases S. Hartmann, D. Weidlich and D. Klostermeier 12. Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET J. Hohlbein and A.N. Kapanidis 13. Methods for Investigating DNA Accessibility with Single Nucleosomes M.D. Gibson, M. Brehove, Y. Luo, J. North and M.G. Poirier 14. Single-Molecule Fluorescence Studies of Fast Protein Folding Z. Wang, L.A. Campos and V. Muñoz 15. Single-Molecule Multi-Color FRET Assay for Studying Structural Dynamics of Biomolecules S. Lee, Y. Jang, S.-J. Lee and S. Hohng 16. A Multi-Color Single Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously M. Götz, P. Wortmann, S. Schmid and T. Hugel 17. Interferometric Scattering Microscopy for the Study of Molecular Motors J. Andrecka, Y. Takagi, K.J. Mickolajczyk, L.G. Lipert, J.R. Sellers, W.O. Hancock, Y.E. Goldman and P. Kukura 18. Enzyme Kinetics in Femtoliter Arrays P. Mogalisetti and D.R. Walt