Liquid Chromatography: Fundamentals and Instrumentation

Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field.

In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more.

This second edition addresses these new developments with updated chapters from the most expert researchers in the field.

Emphasizes the integration of chromatographic methods and sample preparationExplains how liquid chromatography is used in different industrial sectorsCovers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical)Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

1. Milestones in the development of liquid chromatography
2. Kinetic theories of liquid chromatography
3. Column technology in liquid chromatography
4. Reversed-phase liquid chromatography
5. Secondary chemical equilibria in reversed-phase liquid chromatography
6. Hydrophilic interaction chromatography
7. Hydrophobic interaction chromatography
8. Liquid-solid chromatography
9. Ion chromatography
10. Size-exclusion chromatography
11. Interaction polymer chromatography
12. Affinity chromatography
13. Solvent selection in liquid chromatography
14. Method development in liquid chromatography
15. Theory and practice of gradient elution liquid chromatography
16. Comprehensive two-dimensional liquid chromatography
17. General instrumentation in HPLC
18. Advanced spectrometric detectors for identification and quantification: Mass spectrometry
19. Advanced IR and Raman detectors for identification and quantification
20. Advanced spectrometric detectors for identification and quantification: Nuclear magnetic resonance
21. Data analysis
22. Validation of liquid chromatographic methods
23. Quantitative structure property (retention) relationships in liquid chromatography
24. Modeling of preparative liquid chromatography
25. Process concepts in preparative liquid chromatography
26. Miniaturization and microfluidics
27. Nano-liquid chromatography
28. Capillary electrochromatography
29. Ultra-high performance liquid chromatography