Rational Design of Enzyme-Nanomaterials

Rational Design of Enzyme-Nanomaterials, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in rational design of enzyme-nanomaterials, and includes sections on such topics as conjugation of enzymes and dextran-aldehyde polymers, improved activity of enzymes bound to titanate nanosheet, nano-layered 'stable-on-the-table' biocatalysts and nanoparticle-based enzyme sensors.

Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in rational design of enzyme-nanomaterialsContains sections on such topics as conjugation of enzymes and dextran-aldehyde polymers, improved activity of enzymes bound to titanate nanosheet, nano-layered 'stable-on-the-table' biocatalysts, and nanoparticle-based enzyme sensors

1. Preparation of Biocatalytic Microparticles by Interfacial Self-Assembly of Enzyme-Nanoparticle Conjugates Around a Cross-Linkable Core
Stephanie M. Andler, Li-Sheng Wang, Julie M. Goddard and Vincent M. Rotello
2. Monitoring Enzymatic Proteolysis Using Either Enzyme- or Substrate Bioconjugated Quantum Dots
Sebastián A. Díaz , Joyce C. Breger and Igor L. Medintz
3. Intense Pegilation of enzyme surfaces: Relevant Stabilizing Effects
Sonia Moreno-Pérez, Alejandro H. Orrego, María Romero, Lara Trobo-Maseda, Sandro Martins-DeOliveira, Roberto Munilla, Gloria Fernández-Lorente and Jose M. Guisan
4. Immobilization of Lipases on Heterofunctional Octyl-glyoxyl Agarose Supports: Improved Stability and Prevention of the Enzyme Desorption
Nazzoly Rueda, Jose C. S. dos Santos, Rodrigo Torres, Claudia Ortiz, Oveimar Barbosa and Roberto Fernandez-Lafuente
5. Biomimetic/Bioinspired Design of Enzyme@capsule Nano/Micro-Systems
Jiafu Shi, Yanjun Jiang, Shaohua Zhang, Dong Yang and Zhongyi Jiang
6. Synergistic Functions of Enzymes Bound to Semiconducting Layers
Kai Kamada, Misato Kamiuchi, Motoko Tokunaga, Daiki Ito and Nobuaki Soh
7. Bioconjugation of Antibodies and Enzyme Labels onto Magnetic Beads
Brunah A. Otieno, Colleen E. Krause and James F. Rusling
8. Rationally Designed, 'Stable-on-the-Table' nanoBiocatalysts bound to Zr(IV) Phosphate Nanosheets
Inoka K. Deshapriya  and Challa V. Kumar
9. Portable Enzyme-paper Biosensors Based on Redox Active CeO2 Nanoparticles
Anahita Karimi, Ali Othman and Silvana Andreescu
10. Rational Design of Nanoparticle Platforms for ‘Cutting-the-Fat’: Covalent Immobilization of Lipase, Glycerol Kinase and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles
Vishakha Aggarwal and Chandra Shekhar Pundir
11. Biographene: Direct Exfoliation of Graphite in A Kitchen Blender for Enzymology Applications
Challa Vijaya Kumar and Ajith Pattammattel