Mass Spectrometry: Techniques for Structural Characterization of Glycans

Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules.

As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study.

Authored by an analytical chemist with more than 30 years of experience in research and industryServes as a quick reference in mass spectral analysis and carbohydratesIncludes more than 60 figures to aid in the retention of key concepts

• Dedication
• Mass Spectral Analysis of Carbohydrates
  • Abstract
  • 1 Isolation Methods for MS Analysis
  • 2 Component Monosaccharide Analysis by Acid Catalyzed Hydrolysis of Glycan and Qualitative Chromatographic Identification by HPLC
  • 3 Identification of Glycoses Versus Glycitols by HPAEC-PAD Difference Chromatograms
  • 4 The Methods for Preparing an Oligosaccharide Reducing Glycan from a Glycoprotein, Linked Through O-Glycosidic Linkage
  • Conclusions
  • References